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Gene expression profile of embryonic stem cell-derived TS-like cells by RNA-sequencing

GSE284316 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/05/31 Platform GPL18573
Summary
Human embryonic stem cells (ESCs) were differentiated into GATA3-expressing extraembryonic cells (GATA3+ ExE cells) by exposure to chemical inhibitors targeting ACTIVIN/NODAL (A83-01, 1uM) and FGF signaling (PD03714, 100nM). Using these GATA3-expressing cells as a starting material, we established trophoblast stem-like cells (TSLCs) following the established protocol for deriving trophoblast stem cells (TSCs) from the villous trophoblast of human first-trimester placenta. To characterize the resulting ESC-derived TSLCs, RNA sequencing was conducted, allowing comparisons with the parental ESCs and previously published TSCs.
Published in
Stepwise strategy for generating human trophoblast stem-like cells from embryonic stem cells reveals specific role of TFAP2C in acquiring self-renewability
Ohgushi M, Honda K, Takagi R et al. · Scientific reports 2025 · PMID 40447714 · doi:10.1038/s41598-025-03830-x
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Also filed as BioProject PRJNA1198534 and SRA study SRP551547. Searching any of these in the dataset finder brings you back here.

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