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Single-Cell Transcriptomic Profiling of Xenotransplanted Retinal Organoids Derived from the WA09 CRX-tdTomato Human Embryonic Stem Cell Line

GSE285393 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/06/01 Platform GPL30173
Summary
Single-cell gene expression profiles were generated for human retinal organoids derived from the WA09 CRX-tdTomato human embryonic stem cell line. Fragmented organoids were transplanted into the subretinal space of normal canine retinas and analyzed three days post-transplantation. In parallel, fragmented organoids from the same batch, maintained in culture for the same duration, were used as controls. Comparative single-cell transcriptomic analysis provided insights into the early molecular changes associated with the transplanted environment versus in vitro culture conditions, shedding light on the cellular responses and adaptation of retinal organoids post-transplantation.
Published in
Metabolic stress and early cell death in photoreceptor precursor cells following retinal transplantation
Sudharsan R, Dolgova N, Kwok J et al. · Stem cell research & therapy 2025 · PMID 40708039 · doi:10.1186/s13287-025-04509-w
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Direct links to NCBI, no account and no request form: the whole study as GSE285393_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1203270 and SRA study SRP553959. Searching any of these in the dataset finder brings you back here.

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