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Single-cell transcriptomic based heterogeneity of adipose-derived regenerative cells is independent of gender and BMI

GSE286321 Homo sapiens (human) Single-cell RNA-seq 17 samples Submitted 2025/03/12 Platform GPL24676
At a glance

Single-cell RNA-seq · human · 17 samples

What it is
Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism
Homo sapiens (human)
Samples
17 samples: 17 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMIStem cell research & therapy · 2025
Summary (written by the submitters)
Adipose-derived regenerative cells (ADRCs) are implicated in fat homeostasis and metabolism, but also provide an ample source of cells with widespread regenerative therapeutic use. Being heterogenous in nature, possibly affecting clinical outcome, the ADRC donor BMI and gender may have a large impact on ADRC composition and quality but this remains largely unexplored.
Read the full summary ↓
Herein, we provide a comprehensive single-cell RNA sequencing ADRC mapping across two cell trial intervention studies but found no gender- or BMI-related variations, except for a minor female increase in PI16/CD55-expressing stem cells. This provides decision-making support on adipose stem cell donor selection, but also adds to gender-based metabolomics.
Published in
Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMI
Bjerre FA, Nielsen JV, Burton M et al. · Stem cell research & therapy 2025 · PMID 40038777 · doi:10.1186/s13287-025-04234-4
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE286321_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1208674 and SRA study SRP556395. Searching any of these in the dataset finder brings you back here.

Study design
17 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 17 sample titles: 17 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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