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Neuronal activation triggers rapid biphasic FMRP-mediated translational control linking synaptic and nuclear regulation [RiboTag]

GSE286381 Mus musculus (mouse) RNA-seq (gene expression) 32 samples Submitted 2025/01/15 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 32 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
32 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
A central question in protein synthesis–dependent synaptic plasticity is how translation of neuronal mRNAs is dynamically regulated in time and space. Here, we develop Opto-CLIP, integrating optogenetic stimulation with cell-type-specific crosslinking immunoprecipitation (CLIP) of FMRP in CA1 neurons to define activity-dependent RNA regulation.
Read the full summary ↓
Following patterned neuronal activation, we identify dynamic changes in FMRP-mediated translational control at minute-scale resolution. Within five minutes, FMRP is released from synaptic transcripts, accompanied by increased ribosome association. By 30 minutes, FMRP dissociates from transcripts encoding nuclear and RNA regulatory factors, also accompanied by increased ribosome association. At this stage, despite a marked reduction in protein levels, FMRP selectively reassociates with synaptic transcripts, coincident with reduced ribosome association. These coordinated shifts define temporally structured waves of translational control linking synaptic and nuclear programs. Our findings reveal a biphasic program of FMRP-mediated translational control that couples local synaptic responses to delayed nuclear regulation, providing a mechanistic framework linking activity-dependent RNA regulation, its disruption in Fragile X syndrome, and homeostatic plasticity.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE286381_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1209014 and SRA study SRP556507. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 32 more — browse all 32 samples with per-sample file links →

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