Bulk RNA-Seq of primary human bronchial epithelial cells after transfection with different Cas9 formats
RNA-seq (gene expression) · human · 17 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 17 samples: 5 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE287215_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1211640 and SRA study SRP557866. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 14 samples.
How we read this: 5 replicated groups read from 17 sample titles; they account for 14 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8741424 Primary Human Brionchial Epithelial (NHBE) cells, modified mRNA, batch P3515, replicate R_modified_mRNA_1 Primary Human Brionchia…
- GSM8741425 Primary Human Brionchial Epithelial (NHBE) cells, untreated, batch P3515, replicate R_untreated_1 Primary Human Brionchia…
- GSM8741426 Primary Human Brionchial Epithelial (NHBE) cells, modified mRNA, batch P3515, replicate R_modified_mRNA_2 Primary Human Brionchia…
- GSM8741427 Primary Human Brionchial Epithelial (NHBE) cells, untreated, batch P3515, replicate R_untreated_2 Primary Human Brionchia…
- GSM8741428 Primary Human Brionchial Epithelial (NHBE) cells, modified mRNA, batch P3515, replicate R_modified_mRNA_3 Primary Human Brionchia…
- GSM8741429 Primary Human Brionchial Epithelial (NHBE) cells, untreated, batch R378, replicate R_untreated_1
- GSM8741430 Primary Human Brionchial Epithelial (NHBE) cells, RNP, batch R378, replicate R_RNP_1
- GSM8741431 Primary Human Brionchial Epithelial (NHBE) cells, unmodified mRNA, batch R378, replicate R_unmodified_mRNA_1
- GSM8741432 Primary Human Brionchial Epithelial (NHBE) cells, untreated, batch R528, replicate R_untreated_1 Primary Human Brionchia…
- GSM8741433 Primary Human Brionchial Epithelial (NHBE) cells, RNP, batch R528, replicate R_RNP_1 Primary Human Brionchia…
- GSM8741434 Primary Human Brionchial Epithelial (NHBE) cells, unmodified mRNA, batch R528, replicate R_unmodified_mRNA_1 Primary Human Brionchia…
- GSM8741435 Primary Human Brionchial Epithelial (NHBE) cells, untreated, batch R528, replicate R_untreated_2 Primary Human Brionchia…
- GSM8741436 Primary Human Brionchial Epithelial (NHBE) cells, RNP, batch R528, replicate R_RNP_2 Primary Human Brionchia…
- GSM8741437 Primary Human Brionchial Epithelial (NHBE) cells, unmodified mRNA, batch R528, replicate R_unmodified_mRNA_2 Primary Human Brionchia…
- GSM8741438 Primary Human Brionchial Epithelial (NHBE) cells, untreated, batch R528, replicate R_untreated_3 Primary Human Brionchia…
- GSM8741439 Primary Human Brionchial Epithelial (NHBE) cells, RNP, batch R528, replicate R_RNP_3 Primary Human Brionchia…
- GSM8741440 Primary Human Brionchial Epithelial (NHBE) cells, unmodified mRNA, batch R528, replicate R_unmodified_mRNA_3 Primary Human Brionchia…
- GSE304598 Bulk RNA-seq time-course of human iPSc differentiation to thymic epithelial progenitors 33 samples shares epithelial, different, rna-seq
- GSE292944 Culture Media Strongly Influences Primary Human Bronchial Epithelial Cells' Transcriptomic Response to Ozone Exposure 24 samples shares epithelial, bronchial, primary
- GSE201157 Expression profiling by high-throughput sequencing to evaluate effects of aroylated phenylenediamine HO53 on transcriptome in human undifferentiated bronchial epithelial cells (BCi-NS1.1 cell line). 12 samples shares epithelial, bronchial, different
- GSE290621 Quadruple adenine base edited allogeneic CAR T cells outperform CRISPR/Cas9 nuclease engineered T cells [RNA-seq] 33 samples shares cas9, rna-seq
- GSE275647 Role of Cancer-Associated Fibroblasts in Prognostic Stratification of Advanced-Stage Gastric Cancer through Interacting with Endothelial and Malignant Epithelial Cells [AGC RNA-seq] 216 samples shares epithelial, rna-seq
- GSE282444 Epigenetic Memory of Intestinal Epithelial Cells in Inflammatory Bowel Disease [RNA-seq] 56 samples shares epithelial, rna-seq
- GSE161245 Comparison of bronchial epithelial cells obtained by bronchoscopy from mild asthma patients, moderate asthma patients, severe asthma patients and healthy controls 55 samples shares epithelial, bronchial
- GSE238235 The impact of vaping on adolescent lung function and nasal epithelial gene expression [RNA-seq] 48 samples shares epithelial, rna-seq
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.