Expression profiling by high-throughput sequencing to evaluate effects of aroylated phenylenediamine HO53 on transcriptome in human undifferentiated bronchial epithelial cells (BCi-NS1.1 cell line).

We used RNA sequencing to exploit molecular mechanisms behind HO53-induced innate immune responses in undifferentiated human bronchial epithelial cells (BCi-NS1.1 cell line). We performed transcriptome analysis of undifferentiated bronchial epithelial cells (BCi-NS1.1 cell line) treated with aroylated phenylenediamine HO53 for different time points 4 h, 8 h and 24 h and compared it to transcriptome of untreated cells. The broad effect of HO53 on the gene expression in undifferentiated human lung epithelial cells (BCi-NS1.1) points towards an epigenetic event. Furthermore, genes involved in metabolic pathways (glycolysis, TCA cycles, pentose phosphate, glycosylation and gluconeogenesis) were particularly affected by HO53 treatement. The expression pattern for few relevant selected genes was reconfirmed by qRT-PCR using SYBR Green reagent.