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Roles of DNA hydroxymethylation versus DNA formylation and carboxylation in neural stem cells [RNA-seq]

GSE287654 Mus musculus Expression profiling by high throughput sequencing 7 samples Submitted 2025/07/14 Platform GPL24247
Summary
TET enzymes are highly expressed in neural stem cells (NSCs). They oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) in a step-wise manner. In this study we have generated NSCs that only lack the Tet formylation and carboxylation activities (Tet-TFoCa) and have compared their multipotency, transcriptome and epigenome to NSCs that lacked all three Tet activities (Tet-TMut). Our findings reveal unique roles for DNA hydroxymethylation vs DNA formylation and carboxylation in multipotency of NSCs.
Published in
Establishment of neuronal and glial competence of neural stem cells requires distinct enzymatic activities of TET enzymes
Ebert BC, MacArthur IC, Ketchum HC et al. · Stem cell reports 2025 · PMID 40749667 · doi:10.1016/j.stemcr.2025.102595
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Also filed as BioProject PRJNA1214202 and SRA study SRP559236. Searching any of these in the dataset finder brings you back here.

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