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Antigen-specific CD4+ T cells promote monocyte recruitment and differentiation into glycolytic lung macrophages to control Mycobacterium tuberculosis.

GSE287753 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/01/27 Platform GPL34328
Summary
Although lung myeloid cells provide an intracellular niche for Mycobacterium tuberculosis (Mtb), CD4+ T cells limit Mtb growth in these cells to protect the host. Here, we show that monocyte-derived macrophages (MDMs), instead of phenotypically similar dendritic cells, are preferentially infected with Mtb in murine lungs. Mtb-specific CD4+ T cells recruited monocyte precursors of MDMs into the lungs via interferon-γ (IFN-γ). Although the CD4+ T cells increased the number of Mtb-infectable cells in the lungs, they then attenuated Mtb growth by engaging in MHC class II (MHCII)-mediated cognate interactions with monocyte-derived cells to promote their disinfection. Specifically, cognate CD4+ T cell help via MHCII enhanced MDM expression of glycolytic genes independently of IFN-γ. These results indicate that CD4+ T cells recruit infectable MDMs to the lungs and trigger glycolysis-dependent bacterial control within them by engaging MHCII-bound Mtb peptides on their surfaces.
Published in
Antigen-specific CD4+ T cells promote monocyte recruitment and differentiation into glycolytic lung macrophages to control Mycobacterium tuberculosis
Becker SH, Ronayne CE, Bold TD et al. · PLoS pathogens 2025 · PMID 40489538 · doi:10.1371/journal.ppat.1013208
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Also filed as BioProject PRJNA1214388 and SRA study SRP559276. Searching any of these in the dataset finder brings you back here.

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