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Reprogramming breast cancer cells to breast cancer stem cells-like by the POU1F1 transcription factor

GSE287812 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/05/06 Platform GPL34281
Summary
POU1F1 (also named PIT-1) is a well-known transcription factor involved in embryonic and adult pituitary development that has also been analyzed for its role in breast cancer . This study provides compelling evidence of POU1F1 in reprogramming breast cancer cells into BCSC-like. Our data demonstrates that POU1F1 induces a BCSC-like phenotype in breast tumor cells by deregulating markers such as CD24, CD44, CD133, and ALDH. These phenotypic modifications are correlated with functional changes, i.e. higher clonogenicity, increased mammospheres formation, and increased glycolysis metabolism. In addition, using in vivo immunodeficient mice models we found that a subpopulation of ALDHhigh cells from the POU1F1 overexpressing MCF-7 cells possess high tumor-initiating capacity, high resistance to chemo- and radiotherapy, and reduced overall survival. Mechanistically, POU1F1 induces an increase in IL-6 mRNA expression and raises IL-6 in the culture medium. This activates the IL-6/JAK/STAT3 pathway, which in turn increases ALDH mRNA expression.
Published in
POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH
Avila L, Seoane S, Rodriguez-Gonzalez S et al. · NPJ breast cancer 2026 · PMID 41857068 · doi:10.1038/s41523-026-00929-w
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Also filed as BioProject PRJNA1214747 and SRA study SRP559405. Searching any of these in the dataset finder brings you back here.

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