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Engineered biomaterial immunological niches as a surrogate for diseased tissue in chronic progressive EAE

GSE287968 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/01/24 Platform GPL24247
Summary
We use a subcutaneous biomaterial implant as a surrogate for sampling immune cells phenotypes in murine chronic EAE. These implants are microporous polycaprolactone scaffolds that form immunological niches that get vascularized and capture both innate and adaptive immune cells. These niches can be minimally invasively biopsied, allowing for multiple samples to be collected from the same mouse at various timepoints in disease: presymptomatic (Day 7) and early state disease (Day 9). This study investigates immune dynamics over time in subclinical and early stage chronic progressive EAE at a distal biomaterial-based immunological niche.
Published in
Engineered immunological niche directs therapeutic development in models of progressive multiple sclerosis
Rad LM, Hughes KR, Wheeler SN et al. · Proceedings of the National Academy of Sciences of the United States of America 2025 · PMID 39937858 · doi:10.1073/pnas.2409852122
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Also filed as BioProject PRJNA1215376 and SRA study SRP559713. Searching any of these in the dataset finder brings you back here.

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