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Krüppel-like Factor family members control expression of genes required for serous cavity and alveolar macrophage identities [CUT&Run]

GSE288282 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 6 samples Submitted 2025/02/19 Platform GPL24247
Summary
Tissue-resident macrophages adopt distinct gene expression profiles and exhibit functional specialization based on their tissue of residence. Recent studies have begun to define the signals and transcription factors that induce these identities. Here we describe an unexpected and specific role for the broadly expressed transcription factor Kruppel-like Factor 2 (KLF2) in the development of embryonically derived Large Cavity Macrophages (LCM) in the serous cavities. KLF2 not only directly regulates the transcription of genes previously shown to specify LCM identity, such as retinoic acid receptors and GATA6, but also is required for induction of many other transcripts that define the identity of these cells. We identify a similar role for KLF4 in regulating the identity of alveolar macrophages in the lung. These data demonstrate that broadly expressed transcription factors, such as Group 2 KLFs, can play important roles in the specification of distinct identities of tissue-resident macrophages.
Published in
KLF family members control expression of genes required for tissue macrophage identities
Pestal K, Slayden LC, Barton GM · The Journal of experimental medicine 2025 · PMID 40072341 · doi:10.1084/jem.20240379
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Also filed as BioProject PRJNA1216669 and SRA study SRP560488. Searching any of these in the dataset finder brings you back here.

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