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Nascent-seq of HeLa WT and SRSF5 KO cells

GSE288960 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/01/29 Platform GPL24676
Summary
We used HeLa cells where SRSF5 was knocked out by CRISPR/Cas9 to perform Nascent-seq. WT cells served as control. One hour before harvesting, cells were incubated with 4-sU for exactly 1 hour to label newly transcribed RNAs. 4-sU-labelled RNAs were biotinylated and purified over streptavidin beads and transformed into an RNA-seq library. We compared differential expression and intron retention between WTand SRSF5 KO samples.
Published in
Rapid depletion and super-resolution microscopy reveal dual roles of SRSF5 in coordinating nuclear speckle-paraspeckle crosstalk during cellular stress
Okuda EK, Kessler LF, Arnold B et al. · Nucleic acids research 2025 · PMID 40716777 · doi:10.1093/nar/gkaf713
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Also filed as BioProject PRJNA1220103 and SRA study SRP562031. Searching any of these in the dataset finder brings you back here.

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