GEO series

NRF2 deficiency leads to inadequate beta cell adaptation during pregnancy and gestational diabetes

GSE289077 Mus musculus (mouse) RNA-seq (gene expression) 15 samples Submitted 2025/03/07 Platform GPL21103

At a glance

RNA-seq (gene expression) · mouse · 15 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 15 samples: 15 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: NRF2 deficiency leads to inadequate beta cell adaptation during pregnancy and gestational diabetesRedox biology · 2025

Summary (written by the submitters)

The late stages of mammalian pregnancy are accompanied by a mild increase in insulin resistance likely due to enhanced glucose demand of the growing fetus. Therefore, as an adaptive process to maintain euglycemia during pregnancy, maternal β-cell mass expands leading to increased insulin release. Defects in functional β-cell adaptive expansion during pregnancy can lead to gestational diabetes mellitus (GDM).

Read the full summary ↓

While the exact mechanisms that promote GDM are poorly understood, GDM is associated with inadequate functional β-cell mass expansion and with a systematic increase of oxidative stress. Here, we show that NRF2 levels are upregulated in mouse β-cells at gestational day 15 (GD15). Inducible β-cell-specific Nrf2 deleted (βNrf2KO) mice display reduced β-cell proliferation, increased β-cell oxidative stress and lipid peroxidation, compromised β-cell function, and elevated β-cell death, leading to impaired β-cell mass expansion and dysregulated glucose homeostasis towards the end of pregnancy. Importantly, the gestational hormone 17-β-estradiol (E2) increases NRF2 levels, and downregulation of NRF2 suppresses E2-induced protection of β-cells against oxidative stress, suggesting that E2 exerts its antioxidant effects through activation of NRF2 signaling in β-cells. Collectively, these data highlight the critical role of NRF2 in regulating oxidative stress during the adaptive response of β-cells in pregnancy and identify NRF2 as a potential therapeutic target for GDM treatment.

Published in

NRF2 deficiency leads to inadequate beta cell adaptation during pregnancy and gestational diabetes

Haidery F, Lambertini L, Tse I et al. · Redox biology 2025 · PMID 40054060 · doi:10.1016/j.redox.2025.103566

This dataset

Open GSE289077 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE289077_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1221270 and SRA study SRP562371. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40054060) ↗

Study design

15 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 15 sample titles: 15 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8783401 np_control2, pancreatic isltes, non-pregnant, vehicle treatment
GSM8783402 np_control4, pancreatic isltes, non-pregnant, vehicle treatment
GSM8783403 np_control5, pancreatic isltes, non-pregnant, vehicle treatment
GSM8783404 np_control6, pancreatic isltes, non-pregnant, vehicle treatment
GSM8783405 np_Nrf2KO4, pancreatic isltes, Nrf2-KO, tamoxifen treatment
GSM8783406 np_Nrf2KO5, pancreatic isltes, Nrf2-KO, tamoxifen treatment
GSM8783407 np_Nrf2KO6, pancreatic isltes, Nrf2-KO, tamoxifen treatment
GSM8783408 gd15_control1, pancreatic isltes, pregnant (gestational day 15), vehicle treatment
GSM8783409 gd15_control2, pancreatic isltes, pregnant (gestational day 15), vehicle treatment
GSM8783410 gd15_control3, pancreatic isltes, pregnant (gestational day 15), vehicle treatment
GSM8783411 gd15_control4, pancreatic isltes, pregnant (gestational day 15), vehicle treatment
GSM8783412 gd15_Nrf2KO1, pancreatic isltes, pregnant (gestational day 15), tamoxifen treatment
GSM8783413 gd15_Nrf2KO2, pancreatic isltes, pregnant (gestational day 15), tamoxifen treatment
GSM8783414 gd15_Nrf2KO3, pancreatic isltes, pregnant (gestational day 15), tamoxifen treatment
GSM8783415 gd15_Nrf2KO4, pancreatic isltes, pregnant (gestational day 15), tamoxifen treatment

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.