Effects of electrical pulse stimulation and hydrogen peroxide as its byproduct on in vitro contractile skeletal muscle tissue.

The in vitro human skeletal muscle model is a valuable tool for studying muscle physiology and the mechanisms underlying muscle diseases. Tissue engineering approach could produce contractile human muscle tissue, with electrical pulse stimulation (EPS) enhancing its contractile ability. We evaluated transcriptomic changes induced by EPS, two different patterns of EPS were applied to differentiated human myofiber sheets with cultured fibrin-gel. After 3 days of EPS-induced exercise, RNA was extracted from these two differently stimulated samples and non-stimulated control sample and analyzed by RNA-sequencing. For the samples with an excess EPS pattern, catalase was supplemented in the media to degrade hydrogen peroxide generated by EPS, and RNA was extracted from the stress-mitigated sample and also analyzed by RNA-sequencing.