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Identification of two transitional stem-like memory CD8+ T cell states during viral infection and vaccination

GSE289245 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/02/21 Platform GPL30172
Summary
Stem-like CD8+ T cells are a key feature of chronic conditions and long-lived memory. Currently stem-like CD8+ T cells are named according to their environment, where precursors of exhausted (TPEX) cells arise in chronic viral infections, autoimmunity and cancer, while stem cell-like memory (TSCM) cells are associated with acute infection and vaccination. Here, we established and validated a method to monitor the transition between transcriptionally distinct TPEX and TSCM cell states. We reveal a linear and reversible developmental trajectory that is concomitant with viral clearance. Further, we demonstrate that TPEX to TSCM cell conversion is observed during acute viral infection and vaccination, aligning the processes that establish stem-like CD8+ T cells in acute and chronic settings. This work supports the potential to monitor stem-like CD8+ T cell conversion as biomarkers of disease in patients with chronic infection, cancer, and autoimmunity and broadens the importance of this transition during the establishment of vaccine-induced long-lived immune protection.
Published in
Transient inhibition of type I interferon enhances CD8+ T cell stemness and vaccine protection
Broomfield BJ, Tan CW, Qin RZ et al. · The Journal of experimental medicine 2025 · PMID 40062995 · doi:10.1084/jem.20241148
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Also filed as BioProject PRJNA1221968 and SRA study SRP563064. Searching any of these in the dataset finder brings you back here.

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