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Tracking tumor cell direct interactome reveals a proliferating cell layer competition structure for CTRB1-driven epithelial-stromal defense against cancer

GSE289634 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2026/05/20 Platform GPL24676
Summary
Purpose: To explore the molecular mechanism of CTRB1 boosts host endows cell fitness to outcompete tumor cells, RNA sequencing was performed to analyzeand compare the gene signatures of between HEK 293FTGFP and HEK 293FTCTRB1-GFP cells. Methods: Total mRNA was extracted from HEK 293FTGFP and HEK 293FTCTRB1-GFP cells. Then RNA quality was assessed using an Agilent Bioanalyzer 2100 and the sample reads were sequenced using Illumina NovaSeq 6000 platform. Results: Our results revealed differentially expressed genes in HEK 293FTCTRB1-GFP cells when compared with HEK 293FTGFP cells. Further KEGG analysis reveal that expression of CTRB1 influenced several proliferation-related signaling pathways, including Hippo-YAP, WNT, and Notch. Conclusion: Our study present the detailed transcripts analysis of HEK 293FTGFP and HEK 293FTCTRB1-GFP cells. Based on RNA-seq transcriptome characterization, indicating that CTRB1 can activate multiple proliferation-related signaling pathways expression to dictate cell fitness during cell competition.
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Direct links to NCBI, no account and no request form: the whole study as GSE289634_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1223866 and SRA study SRP563984. Searching any of these in the dataset finder brings you back here.

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