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Supplement and refinement the standard and analysis of primary human hepatocytes.

GSE289636 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/09/02 Platform GPL24676
Summary
We use high-throughout single-cell RNA sequencing technology to analyze primary human hepatocytes, combining with LC-MS/MS for drug-metabolizing function research. We measure the enzymatic metabolic function of PHH and perform a correlation analysis with the values documented in the quality inspection report. Furthermore, we assess functions beyond cellular metabolism as specified in the PHH standards. These additional functions include bile secretion, albumin (ALB) secretion, and cell purity, which are not addressed in the quality inspection report. Additionally, we examine the impact of donor characteristics such as body weight, age, underlying health conditions, and lifestyle habits (including smoking and alcohol consumption) on cellular function. We aim to conduct a comprehensive analysis of the factors that influence cellular function. This research not only seeks to supplement and refine the standards of PHH but also aspires to provide a crucial reference for the application of PHH in the domains of disease research and drug development in the future.
Published in
The validation of quality attributes in Primary Human Hepatocytes Standard
Peng Z, Wu J, Zhang X et al. · Cell regeneration (London, England) 2025 · PMID 41100036 · doi:10.1186/s13619-025-00258-6
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Direct links to NCBI, no account and no request form: the whole study as GSE289636_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1223864 and SRA study SRP563999. Searching any of these in the dataset finder brings you back here.

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