GEO series

GH-resistant (Laron) mice: gene therapy with a liver-specific GH receptor causes unbalanced upregulation of female-biased and growth-related genes

GSE289769 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2026/06/18 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 24 samples: 24 × KC14Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: GH-resistant (Laron) mice: gene therapy with a liver-specific GH receptor causes unbalanced upregulation of female-biased and growth-related genesFrontiers in endocrinology · 2026

Summary (written by the submitters)

Growth hormone (GH) receptor (GHR) mutations give rise to GH-resistance (Laron syndrome). We previously treated GH-resistant Ghr-/- mice (Laron mice) with adeno-associated virus (AAV) delivering mouse (m)Ghr controlled by a constitutively active liver-specific promoter (HLP). A single injection of AAV-HLP-mGHR resulted in a significant but limited increase in body length and weight, consistent with studies of IGF-1 treatment in humans and mice.

Read the full summary ↓

Here, we performed RNA-seq on male and female mouse livers comprising the following groups: GHR+/+ (wild-type), GHR-/- (Laron), AAV-HLP-mGHR-treated GHR-/- (treatment group), and AAV-HLP-Luc (Luciferase)-treated GHR-/- (control group). Only four genes showed significant differential expression in GHR -/- mouse liver following Luciferase vector treatment, indicating minimal effect of the AAV-HLP vector. AAV-HLP-mGHR stimulated significant expression changes in 448 genes compared to AAV-HLP-Luc control, substantially fewer than the 2781 genes whose expression was altered in GHR-/- compared to GHR+/+. AAV-HLP-mGHR treatment induced the GH-responsive IGF signaling genes Igf1 and Igfals ~16-fold compared to AAV-HLP-Luc control, but only to 40–45% of GHR+/+ liver levels. The treatment also upregulated a small subset of genes beyond GHR+/+ expression levels (p-adj < 0.05), including the proto-oncogenes Ascl1, Tmprss4, and others. Finally, genes dysregulated upon GHR loss and upregulated in livers of AAV-HLP-mGHR-treated mice were significantly enriched for sex-biased genes, consistent with the major role of GH and GHR in regulating liver sex differences. While gene replacement therapy is a potential therapy for Laron syndrome, an unregulated constitutively active promoter may drive unexpected and unbalanced changes in liver gene expression that will require monitoring.

Published in

GH-resistant (Laron) mice: gene therapy with a liver-specific GH receptor causes unbalanced upregulation of female-biased and growth-related genes

Tay JK, Sia KC, Mohd Rodhi SH et al. · Frontiers in endocrinology 2026 · PMID 42290866 · doi:10.3389/fendo.2026.1808977

This dataset

Open GSE289769 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE289769_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1224752 and SRA study SRP564355. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42290866) ↗

Study design

24 × KC14

Supports a between-group comparison across 24 samples.

How we read this: 1 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8798231 AAV8-HLP-Luc (4E10 vg), Male: KC14-001 KC14
GSM8798232 AAV8-HLP-Luc (4E10 vg), Male: KC14-002 KC14
GSM8798233 AAV8-HLP-Luc (4E10 vg), Male: KC14-005 KC14
GSM8798234 AAV8-HLP-mGHR (4E10 vg), Male: KC14-007 KC14
GSM8798235 AAV8-HLP-mGHR (4E10 vg), Male: KC14-008 KC14
GSM8798236 AAV8-HLP-mGHR (4E10 vg), Male: KC14-012 KC14
GSM8798237 Uninjected GHR+/+ (WT), Male: KC14-101 KC14
GSM8798238 Uninjected GHR+/+ (WT), Male: KC14-102 KC14
GSM8798239 Uninjected GHR+/+ (WT), Male: KC14-103 KC14
GSM8798240 Uninjected GHR-/- (KO), Male: KC14-113 KC14
GSM8798241 Uninjected GHR-/- (KO), Male: KC14-114 KC14
GSM8798242 Uninjected GHR-/- (KO), Male: KC14-136 KC14
GSM8798243 AAV8-HLP-Luc (4E10 vg), Female: KC14-015 KC14
GSM8798244 AAV8-HLP-Luc (4E10 vg), Female: KC14-016 KC14
GSM8798245 AAV8-HLP-Luc (4E10 vg), Female: KC14-017 KC14
GSM8798246 AAV8-HLP-mGHR (4E10 vg), Female: KC14-019 KC14
GSM8798247 AAV8-HLP-mGHR (4E10 vg), Female: KC14-022 KC14
GSM8798248 AAV8-HLP-mGHR (4E10 vg), Female: KC14-024 KC14
GSM8798249 Uninjected GHR+/+ (WT), Female: KC14-119 KC14
GSM8798250 Uninjected GHR+/+ (WT), Female: KC14-120 KC14
GSM8798251 Uninjected GHR+/+ (WT), Female: KC14-121 KC14
GSM8798252 Uninjected GHR-/- (KO), Female: KC14-131 KC14
GSM8798253 Uninjected GHR-/- (KO), Female: KC14-132 KC14
GSM8798254 Uninjected GHR-/- (KO), Female: KC14-134 KC14

Datasets on a similar topic

GSE297142 Transcription profiling of sorted beta cells from Liver-specific insulin receptor knock-out (LIRKO) mice 18 samples shares liver-specific, receptor
GSE237801 Investigation of the usefulness of liver-specific deconvolution method toward legacy data utilization 57 samples shares liver-specific
GSE279351 Liver-specific inactivation of Cideb improves metabolic profiles and ameliorates steatohepatitis and fibrosis in animal models for MASH 45 samples shares liver-specific
GSE294969 Enhanced Expression of the Female-Biased Gene Gsta2 in Male Proximal Tubule Cells Improves Renal Resilience to Ischemia-Reperfusion Injury 30 samples shares female-biased
GSE284601 A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice 24 samples shares liver-specific
GSE312061 Liver-specific delivery of MDM2 antisense oligonucleotides counteracts diet-induced metabolic dysfunction-associated steatotic liver diseases 18 samples shares liver-specific
GSE317653 Liver-specific loss of the transcriptional coregulator ARGLU1 protects against diet-induced obesity in mice through decreased lipid absorption. 16 samples shares liver-specific
GSE246706 Liver-specific matrin-3 knockout alters the hepatic transcriptome of high-fat diet-fed mice [RNA-seq] 16 samples shares liver-specific

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.