GEO series

Splenic nerves NE-β2AR axis exacerbate septic acute kidney injury via modulating neutrophilic immunosuppression [RNA-seq]

GSE289832 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/02/17 Platform GPL28457

At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 12 samples: 3 × WT mice,neutrophil+LPS, vs 3 × WT mice,neutrophil+NE+LPS, vs 3 × ADRB2-KO mice,neutrophil+LPS, vs 3 × ADRB2-KO mice,neutrophil+NE+LPS,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Sepsis is characterized by chronic inflammation and immunosuppression, two immune states that hinder effective infection control and significantly contribute to renal pathological damage. Recent studies have underscored the crucial role of splenic neuroimmune interactions in immune response regulation. However, the precise mechanisms and functions of splenic nerve regulation of splenic immune responses in sepsis-associated acute kidney injury (SA-AKI) remain obscure.

Read the full summary ↓

Our research suggests that preemptive Splenic Denervation in mice can mitigate sepsis-induced renal injury, while local norepinephrine injection into the spleen exacerbates sepsis-induced kidney damage in the cecal ligation and puncture (CLP) mouse model. Targeted blockade of splenic β2-adrenergic receptor signaling leads to increased survival rates and diminished renal damage in the mice treated with CLP. Subsequent experiments involving local splenic administration of Gr-1 antibodies and myeloid cell-specific conditional knockout of Adrb2 in mice validate that during sepsis, NE/β2-AR signaling can impact splenic neutrophils, fostering the progression of acute sepsis. Mechanistic inquiries reveal that splenic Adrb2 signaling boosts neutrophil PGE2 signaling through the cAMP pathway, promoting Th1 activation, diminishing local bacterial infections, and ameliorating SA-AKI. Furthermore, Blocking ADRB2 or PGE2 synthesizing signal significantly modulates the immunosuppression effect of neutrophils, promoting anti-bacterial Th1 immune response. These studies elucidate the role of splenic NE-ADRB2 signaling in regulating neutrophil immunosuppressive functions, thereby influencing the advancement of sepsis and renal tissue damage.

This dataset

Open GSE289832 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE289832_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1224980 and SRA study SRP564495. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

3 × WT mice,neutrophil+LPS, vs 3 × WT mice,neutrophil+NE+LPS, vs 3 × ADRB2-KO mice,neutrophil+LPS, vs 3 × ADRB2-KO mice,neutrophil+NE+LPS,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8799939 WT mice,neutrophil+LPS,rep1 WT mice,neutrophil+LPS,
GSM8799940 WT mice,neutrophil+LPS,rep2 WT mice,neutrophil+LPS,
GSM8799941 WT mice,neutrophil+LPS,rep3 WT mice,neutrophil+LPS,
GSM8799942 WT mice,neutrophil+NE+LPS,rep1 WT mice,neutrophil+NE+L…
GSM8799943 WT mice,neutrophil+NE+LPS,rep2 WT mice,neutrophil+NE+L…
GSM8799944 WT mice,neutrophil+NE+LPS,rep3 WT mice,neutrophil+NE+L…
GSM8799945 ADRB2-KO mice,neutrophil+LPS,rep1 ADRB2-KO mice,neutrophi…
GSM8799946 ADRB2-KO mice,neutrophil+LPS,rep2 ADRB2-KO mice,neutrophi…
GSM8799947 ADRB2-KO mice,neutrophil+LPS,rep3 ADRB2-KO mice,neutrophi…
GSM8799948 ADRB2-KO mice,neutrophil+NE+LPS,rep1 ADRB2-KO mice,neutrophi…
GSM8799949 ADRB2-KO mice,neutrophil+NE+LPS,rep2 ADRB2-KO mice,neutrophi…
GSM8799950 ADRB2-KO mice,neutrophil+NE+LPS,rep3 ADRB2-KO mice,neutrophi…

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.