← BioTransfer GEO Dataset Finder
GEO series

NRF2 Engenders Anti-inflammatory Stress Macrophages

GSE290106 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/10/10 Platform GPL34290
Summary
We evaluated the function of NRFhigh stress-TAMs and how they respond to immunostimulatory cues. scRNA-seq of CD45+ tumor-infiltrating leukocytes from Keap1flox/flox VavCre and WT mice (n=2 per group) revealed that macrophages were the most abundant leukocyte population in both genotypes. Differential expression, gene set enrichment analysis (GSEA), and cell-by-cell functional scoring indicated a pronounced upregulation of oxidative-stress defense and metabolic adaptation in Keap1 KO macrophages, accompanied by reduced MHC II expression, suppressed interferon-response pathways, and impaired antigen presentation capacity. Despite major shifts in oxidative stress defense and antigen presentation, key macrophage-defining functions such as phagocytic capacity remained comparable between WT and Keap1 KO TAMs. Transcription factor motif analysis further identified NRF2 as a top activated factor and STAT1 as a repressed factor in Keap1 KO TAMs.
Published in
Stress-NRF2 response axis polarizes tumor macrophages and undermines immunotherapy
Schaer DJ, Schulthess-Lutz N, Baselgia L et al. · Journal for immunotherapy of cancer 2025 · PMID 41176316 · doi:10.1136/jitc-2025-013063
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE290106_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1226219 and SRA study SRP565307. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.