← BioTransfer GEO Dataset Finder
GEO series

Effects of WDFY3 overexpression on the transcriptomic profile of murine bone-marrow derived macrophages.

GSE290443 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2025/07/20 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
10 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in miceNature communications · 2025
Summary (written by the submitters)
We determined the transcriptomic profile of bone-marrow derived macrophages (BMDMs) from mice overexpressing human WDFY3 and their wild-type littermate control mice. Specifically, these mice were engineered by knocking a construct consisting of the cDNA sequence encoding a 3x FLAG tag fused to human WDFY3 cDNA, preceded by a STOP cassette flanked by loxP sites, into the Rosa26 locus (Rosa26 loxP-STOP-loxP-3xFLAG-hWDFY3/+), with expression driven by the endogenous Rosa26 promoter.
Read the full summary ↓
To achieve constitutive overexpression, male mice carrying the floxed allele were crossed with female carriers of the HprtCre strain (Stock No. 004302, The Jackson Laboratory), a Cre-deleter strain with 100% Cre-mediated recombination in oocytes, to generate heritable deletion of the loxP-STOP-loxP cassette (Rosa26 3xFLAG-hWDFY3/+). These Rosa26 3xFLAG-hWDFY3/+ mice were interbred to produce homozygotes (Rosa26 3xFLAG-hWDFY3/3xFLAG-hWDFY3) for knock-in (KI) of 3xFLAG-hWDFY3 (hWDFY3KI/KI) and wild-type littermate controls (Ctrl).
Published in
Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice
Wu X, Wang Z, Croce KR et al. · Nature communications 2025 · PMID 41027899 · doi:10.1038/s41467-025-63741-3
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE290443_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1228030 and SRA study SRP566213. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 10 more — browse all 10 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.