GEO series

Testing the contribution of FGL1 to the pathogenesis of metabolic dysfunction-associated steatotic liver disease and hepatocellular carcinoma

GSE291163 Mus musculus (mouse) RNA-seq (gene expression) 16 samples Submitted 2025/08/27 Platform GPL28457

At a glance

RNA-seq (gene expression) · mouse · 16 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 16 samples: 8 × Liver, Fed, WT vs 8 × Liver, Fed, LKOGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Ablation of Hepatocyte Derived-FGL1 Does Not Aggravate Metabolic Dysfunction-Associated Steatotic Liver DiseaseFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025

Summary (written by the submitters)

Metabolic dysfunction-associated steatotic liver disease (MASLD) begins with simple steatosis, which can progress to hepatocellular carcinoma (HCC). The pathogenesis of MASLD alters the secretion of hepatokines such as fibrinogen-like 1 (FGL1), a candidate mediator of liver steatosis and hyperglycemia. To investigate the contribution of FGL1 to liver diseases, we compared wild-type mice to mice with hepatocyte-specific deletion of Fgl1 subjected to a steatosis or HCC experimental protocol.

Read the full summary ↓

We found that mice deficient for Fgl1 in hepatocytes showed higher levels of plasma glucose, pronounced metabolic alterations and liver injury when fed a western diet compared to their wild-type counterparts. However, both genotypes exhibited a similar lipid deposition in the liver. Similarly, wild type and Fgl1-deficient mice displayed comparable liver alterations during HCC progression. We observed that FGL1 expression was repressed during MASLD progression in mice and human concomitantly with the severity of liver injury. Altogether, these findings suggest that FGL1 is not a major contributor to the pathogenesis of MASLD and HCC.

Published in

Ablation of Hepatocyte Derived-FGL1 Does Not Aggravate Metabolic Dysfunction-Associated Steatotic Liver Disease

Personnaz J, Cannizzo L, Martin CMP et al. · FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 · PMID 40832769 · doi:10.1096/fj.202501997R

This dataset

Open GSE291163 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE291163_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1232123 and SRA study SRP568093. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40832769) ↗

Study design

8 × Liver, Fed, WT vs 8 × Liver, Fed, LKO

Supports a between-group comparison across 16 samples.

How we read this: 2 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8830250 Liver, Fed, WT 1 Liver, Fed, WT
GSM8830251 Liver, Fed, WT 2 Liver, Fed, WT
GSM8830252 Liver, Fed, WT 3 Liver, Fed, WT
GSM8830253 Liver, Fed, WT 4 Liver, Fed, WT
GSM8830254 Liver, Fed, WT 5 Liver, Fed, WT
GSM8830255 Liver, Fed, WT 6 Liver, Fed, WT
GSM8830256 Liver, Fed, WT 7 Liver, Fed, WT
GSM8830257 Liver, Fed, WT 8 Liver, Fed, WT
GSM8830258 Liver, Fed, LKO 1 Liver, Fed, LKO
GSM8830259 Liver, Fed, LKO 2 Liver, Fed, LKO
GSM8830260 Liver, Fed, LKO 3 Liver, Fed, LKO
GSM8830261 Liver, Fed, LKO 4 Liver, Fed, LKO
GSM8830262 Liver, Fed, LKO 5 Liver, Fed, LKO
GSM8830263 Liver, Fed, LKO 6 Liver, Fed, LKO
GSM8830264 Liver, Fed, LKO 7 Liver, Fed, LKO
GSM8830265 Liver, Fed, LKO 8 Liver, Fed, LKO

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GSE318801 P21+TREM2+-Senescent Macrophages Fuel Inflammaging and Metabolic Dysfunction-Associated Steatotic Liver Disease. 20 samples shares dysfunction-associated, steatotic, liver
GSE312061 Liver-specific delivery of MDM2 antisense oligonucleotides counteracts diet-induced metabolic dysfunction-associated steatotic liver diseases 18 samples shares dysfunction-associated, steatotic, liver
GSE318804 P21+TREM2+-Senescent Macrophages Fuel Inflammaging and Metabolic Dysfunction-Associated Steatotic Liver Disease. [macro_senescence] 12 samples shares dysfunction-associated, steatotic, liver

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.