Radiation and anti-PD-L1 synergize by stimulating a stem-like T cell population in the tumor-draining lymph node
RNA-seq (gene expression) · mouse · 19 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 19 samples: 5 × sorted CD8+ PD-1+ CD44+ cells; control LN vs 5 × sorted CD8+ PD-1+ CD44+ cells; control Left_tumo vs 5 × sorted CD8+ PD-1+ CD44+ cells; control Right_tumoGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Combination radiation and αPD-L1 enhance tumor control by stimulating CD8+ PD-1+ TCF-1+ T cells in the tumor-draining lymph nodeNature communications · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE291836_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1235601. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 15 samples.
How we read this: 3 replicated groups read from 19 sample titles; they account for 15 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8087003 sorted CD8+ PD-1+ T cells; control
- GSM8087004 sorted CD8+ PD-1+ T cells 7 days after treatment with RT alone
- GSM8087005 sorted CD8+ PD-1+ T cells 7 days after treatment with anti-PDL1 alone
- GSM8087006 sorted CD8+ PD-1+ T cells 7 days after treatment with combination RT and anti-PDL1
- GSM8843059 sorted CD8+ PD-1+ CD44+ cells; control LN1 sorted CD8+ PD-1+ CD44+…
- GSM8843060 sorted CD8+ PD-1+ CD44+ cells; control LN2 sorted CD8+ PD-1+ CD44+…
- GSM8843061 sorted CD8+ PD-1+ CD44+ cells; control LN3 sorted CD8+ PD-1+ CD44+…
- GSM8843062 sorted CD8+ PD-1+ CD44+ cells; control LN4 sorted CD8+ PD-1+ CD44+…
- GSM8843063 sorted CD8+ PD-1+ CD44+ cells; control LN5 sorted CD8+ PD-1+ CD44+…
- GSM8843064 sorted CD8+ PD-1+ CD44+ cells; control Left_tumor1 sorted CD8+ PD-1+ CD44+…
- GSM8843065 sorted CD8+ PD-1+ CD44+ cells; control Left_tumor2 sorted CD8+ PD-1+ CD44+…
- GSM8843066 sorted CD8+ PD-1+ CD44+ cells; control Left_tumor3 sorted CD8+ PD-1+ CD44+…
- GSM8843067 sorted CD8+ PD-1+ CD44+ cells; control Left_tumor4 sorted CD8+ PD-1+ CD44+…
- GSM8843068 sorted CD8+ PD-1+ CD44+ cells; control Left_tumor5 sorted CD8+ PD-1+ CD44+…
- GSM8843069 sorted CD8+ PD-1+ CD44+ cells; control Right_tumor1 sorted CD8+ PD-1+ CD44+…
- GSM8843070 sorted CD8+ PD-1+ CD44+ cells; control Right_tumor2 sorted CD8+ PD-1+ CD44+…
- GSM8843071 sorted CD8+ PD-1+ CD44+ cells; control Right_tumor3 sorted CD8+ PD-1+ CD44+…
- GSM8843072 sorted CD8+ PD-1+ CD44+ cells; control Right_tumor4 sorted CD8+ PD-1+ CD44+…
- GSM8843073 sorted CD8+ PD-1+ CD44+ cells; control Right_tumor5 sorted CD8+ PD-1+ CD44+…
- GSE184661 Lymph node stromal cells support the maturation of pre-DCs into cDCs via colony stimulating factor 1 27 samples shares stimulating, lymph
- GSE249704 Transcriptional profiles of tumor-draining lymph node resident CD11c+ cells in wildtype versus APOE-KO mice 12 samples shares tumor-draining, lymph
- GSE267535 TLR4-dependent fibroblast-monocyte axis in tumor-draining lymph nodes contributes to metastasis in triple-negative breast cancer 10 samples shares tumor-draining, lymph
- GSE328461 Osteopontin mediates acquired resistance to hypoxia-inducing antiangiogenics and promotes anti-PD-L1 refractoriness in breast cancer models 37 samples shares anti-pd-l1
- GSE278396 Cathepsin L as a dual-target to mitigate muscle wasting while enhancing anti-tumor efficacy of anti-PD-L1 [Bulk2] 15 samples shares anti-pd-l1
- GSE278013 Cathepsin L as a dual-target to mitigate muscle wasting while enhancing anti-tumor efficacy of anti-PD-L1 [Bulk1] 14 samples shares anti-pd-l1
- GSE241539 Osteopontin links acquired resistance to hypoxia-inducing antiangiogenics with refractoriness to anti-PD-L1 agents in breast cancer 13 samples shares anti-pd-l1
- GSE186037 Gene expression changes in mouse kidney cancer cells (RENCA) after anti-PD-L1 resistance 12 samples shares anti-pd-l1
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.