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CITE-Seq analysis of intratumoral immune cells from YUMM1.7 melanoma treated with anti-PD-1 therapy.

GSE292038 Mus musculus Expression profiling by high throughput sequencing; Other 6 samples Submitted 2026/03/15 Platform GPL24247
Summary
Metastatic melanoma remains a disease difficult to treat, as 50% of the patients are resistant to immunotherapies or experience high toxicities. Investigations to identify underlying mechanisms focus on T cells or the immunosuppressive tumormicroenvironment (TME). Tumor-associated macrophages (TAMs) make up a large fraction of the TME and high levels of TAM have been associated with poor survival of cancer patients. In this study, the subcutaneous YUMM1.7 melanoma model was used to investigate the responsiveness to anti-PD-1 therapy. The tumor cell line was injected into GRf/f and GRf/f-LysMCre mice to enable insights into glucocorticoid-dependent effects within the myeloid compartment and how they contribute to the responsiveness to anti-PD-1 immunotherapy. Therefore, we profiled sorted CD45+ immune cells from tumors of GRf/f and GRf/f-LysMCre treated with anti-PD-1 therapy or an isotype control antibody via the cellular indexing of transcriptomes and epitomes-method (CITE-Seq).
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Direct links to NCBI, no account and no request form: the whole study as GSE292038_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1236367 and SRA study SRP570546. Searching any of these in the dataset finder brings you back here.

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