GEO series

Temporal impact of sepsis on Alzheimer's disease pathology and neuroinflammation

GSE292277 Mus musculus (mouse) RNA-seq (gene expression) 48 samples Submitted 2025/05/31 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 48 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 48 samples: 16 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Temporal impact of sepsis on Alzheimer's disease pathology and neuroinflammationProgress in neurobiology · 2025

Summary (written by the submitters)

Epidemiological evidence has reported an associative link between sepsis survivorship and increased risk of dementia, particularly Alzheimer's disease (AD). Paradoxically, population studies show females are less susceptible to sepsis but more vulnerable to post-sepsis dementia, including Alzheimer's disease (AD). Here, we examined the temporal impacts of sepsis on AD pathology using an AD-model (TgCRND8) and wild-type mice, assessing outcomes at 7 days and 3 months post-sepsis.

Read the full summary ↓

Following 7-days recovery, all male and female post-septic mice showed robust systemic immune activation. At this time, the female AD-model mice accumulated higher hippocampal amyloid-beta (Aβ) and upregulated AD-type transcriptomic signature. On the other hand, male AD-model mice showed no Aβ changes. Notably, wild-type post-septic males, but not females, displayed robust astrocytosis, with little change in microglial proliferation. By 3 months post-sepsis, microgliosis was specifically elevated in wild-type females, indicating a prolonged central immune response. At this time, both male and female AD-model mice showed exacerbated Aβ and anxiety. Gene network analysis revealed a stronger female immune response, while the male response was linked to estrogen receptor (ESR) signaling, with increased ERα protein observed in the brains of post-septic AD males. Together, our data highlights a sex-dimorphic temporal response in post-sepsis neuroinflammation, with ESR signaling playing a key role in males, while Aβ burden is affected similarly in both males and females.

Published in

Temporal impact of sepsis on Alzheimer's disease pathology and neuroinflammation

Vo Q, Nacionales DC, McFarland KN et al. · Progress in neurobiology 2025 · PMID 40324581 · doi:10.1016/j.pneurobio.2025.102775

This dataset

Open GSE292277 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE292277_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1237815 and SRA study SRP571335. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40324581) ↗

Study design

16 conditions, mostly in triplicate

NTg, Female, sepsis, 3 mon ×3 NTg, Female, sepsis, 7d ×3 NTg, Female, sham, 3 mon ×3 NTg, Female, sham, 7d ×3 NTg, Male, sepsis, 3 mon ×3 NTg, Male, sepsis, 7d ×3 NTg, Male, sham, 3 mon ×3 NTg, Male, sham, 7d ×3 +8 more

Supports a between-group comparison across 48 samples.

How we read this: 16 replicated groups read from 48 sample titles; they account for 48 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8855354 NTg, Female, sepsis, 3 mon - 1 NTg, Female, sepsis, 3 …
GSM8855355 NTg, Female, sepsis, 3 mon - 2 NTg, Female, sepsis, 3 …
GSM8855356 NTg, Female, sepsis, 3 mon - 3 NTg, Female, sepsis, 3 …
GSM8855357 NTg, Female, sepsis, 7d - 1 NTg, Female, sepsis, 7d
GSM8855358 NTg, Female, sepsis, 7d - 2 NTg, Female, sepsis, 7d
GSM8855359 NTg, Female, sepsis, 7d - 3 NTg, Female, sepsis, 7d
GSM8855360 NTg, Female, sham, 3 mon - 1 NTg, Female, sham, 3 mon
GSM8855361 NTg, Female, sham, 3 mon - 2 NTg, Female, sham, 3 mon
GSM8855362 NTg, Female, sham, 3 mon - 3 NTg, Female, sham, 3 mon
GSM8855363 NTg, Female, sham, 7d - 1 NTg, Female, sham, 7d
GSM8855364 NTg, Female, sham, 7d - 2 NTg, Female, sham, 7d
GSM8855365 NTg, Female, sham, 7d - 3 NTg, Female, sham, 7d
GSM8855366 NTg, Male, sepsis, 3 mon - 1 NTg, Male, sepsis, 3 mon
GSM8855367 NTg, Male, sepsis, 3 mon - 2 NTg, Male, sepsis, 3 mon
GSM8855368 NTg, Male, sepsis, 3 mon - 3 NTg, Male, sepsis, 3 mon
GSM8855369 NTg, Male, sepsis, 7d - 1 NTg, Male, sepsis, 7d
GSM8855370 NTg, Male, sepsis, 7d - 2 NTg, Male, sepsis, 7d
GSM8855371 NTg, Male, sepsis, 7d - 3 NTg, Male, sepsis, 7d
GSM8855372 NTg, Male, sham, 3 mon - 1 NTg, Male, sham, 3 mon
GSM8855373 NTg, Male, sham, 3 mon - 2 NTg, Male, sham, 3 mon
GSM8855374 NTg, Male, sham, 3 mon - 3 NTg, Male, sham, 3 mon
GSM8855375 NTg, Male, sham, 7d - 1 NTg, Male, sham, 7d
GSM8855376 NTg, Male, sham, 7d - 2 NTg, Male, sham, 7d
GSM8855377 NTg, Male, sham, 7d - 3 NTg, Male, sham, 7d
GSM8855378 Tg, Female, sepsis, 3 mon - 1 Tg, Female, sepsis, 3 m…
GSM8855379 Tg, Female, sepsis, 3 mon - 2 Tg, Female, sepsis, 3 m…
GSM8855380 Tg, Female, sepsis, 3 mon - 3 Tg, Female, sepsis, 3 m…
GSM8855381 Tg, Female, sepsis, 7d - 1 Tg, Female, sepsis, 7d
GSM8855382 Tg, Female, sepsis, 7d - 2 Tg, Female, sepsis, 7d
GSM8855383 Tg, Female, sepsis, 7d - 3 Tg, Female, sepsis, 7d
GSM8855384 Tg, Female, sham, 3 mon - 1 Tg, Female, sham, 3 mon
GSM8855385 Tg, Female, sham, 3 mon - 2 Tg, Female, sham, 3 mon
GSM8855386 Tg, Female, sham, 3 mon - 3 Tg, Female, sham, 3 mon
GSM8855387 Tg, Female, sham, 7d - 1 Tg, Female, sham, 7d
GSM8855388 Tg, Female, sham, 7d - 2 Tg, Female, sham, 7d
GSM8855389 Tg, Female, sham, 7d - 3 Tg, Female, sham, 7d
GSM8855390 Tg, Male, sepsis, 3 mon - 1 Tg, Male, sepsis, 3 mon
GSM8855391 Tg, Male, sepsis, 3 mon - 2 Tg, Male, sepsis, 3 mon
GSM8855392 Tg, Male, sepsis, 3 mon - 3 Tg, Male, sepsis, 3 mon
GSM8855393 Tg, Male, sepsis, 7d - 1 Tg, Male, sepsis, 7d

+ 8 more — browse all 48 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.