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An amphiregulin reporter mouse enables transcriptional and clonal expansion analysis of reparative lung Treg cells

GSE292440 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/05/14 Platform GPL24247
Summary
Treg cells play critical roles in tissue repair processes, certain of which are mediated by production of the growth factor amphiregulin (Areg). We developed a knock-in mouse model for detection of Areg production in live Treg cells, inserting a Thy1a (Thy1.1) construct into the endogenous Areg locus, such that for every transcript of Areg that is translated, a Thy1.1 transcript is also translated and trafficked separately to the cell surface. We used this mouse model to sort for Thy1.1- or Thy1.1+ (Areg-producing or -non-producing) lung Treg cells in models of lung damage (influenza A virus infection and bleomycin acute lung injury), and used them for assessment of gene expression and clonal expansion of these populations.
Published in
An amphiregulin reporter mouse enables transcriptional and clonal expansion analysis of reparative lung Tregs
Loffredo LF, Kaiser KA, Kornberg A et al. · JCI insight 2025 · PMID 40626358 · doi:10.1172/jci.insight.187245
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Also filed as BioProject PRJNA1238767 and SRA study SRP571834. Searching any of these in the dataset finder brings you back here.

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