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Shared cellular characteristics of dysfunctional stromal cell differentiation underpin impaired healing in two models of perturbed fracture healing

GSE292578 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/01/10 Platform GPL24247
Summary
10% of the fifteen million bone fractures each year that occur in the US fail to heal, which leads to significant loss of function, morbidity, and mortality. However, very little is known about the cellular and molecular mechanisms underpinning inferior healing. Two perturbed fracture models, surgically induced ischemia and the genetic deletion of the self-antigen CD47, were studied to identify unifying cellular and molecular mechanisms. C57Bl/6 wild-type (WT) mice with intact vasculature (WT Intact), WT mice with induced ischemia (WT Ischemic), and CD47-null mice with intact vasculature (CD47-KO) underwent transverse tibial fractures. Single-cell RNA-sequencing (scRNAseq) was performed during the inflammatory stage (4 days post-fracture) and the stromal stage (7 days post fracture) to assess transcriptomic alterations in perturbed fracture calluses.
Published in
Inhibition of CDK8 rescues impaired ischemic fracture healing
Capobianco CA, Song MJ, Farrell EC et al. · NPJ Regenerative medicine 2026 · PMID 41547960 · doi:10.1038/s41536-026-00456-z
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Also filed as BioProject PRJNA1240242 and SRA study SRP572205. Searching any of these in the dataset finder brings you back here.

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