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Targeting the CD40 Co-stimulatory receptor to improve virotherapy efficacy in diffuse midline gliomas

GSE292938 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2025/05/29 Platform GPL30172
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Targeting the CD40 costimulatory receptor to improve virotherapy efficacy in diffuse midline gliomasCell reports. Medicine · 2025
Summary (written by the submitters)
Diffuse Midline Glioma (DMG) is a devastating pediatric brain tumor. The oncolytic adenovirus Delta-24-RGD has shown promising efficacy and safety in DMG patients but is not yet curative. Thus, we hypothesized that activating dendritic cells through the CD40 costimulatory receptor could increase antigen presentation and enhance the anti-tumor effect of the virus, resulting in long-term responses.
Read the full summary ↓
This study showed that the intratumoral co-administration of Delta-24-RGD and a CD40 agonistic antibody is well tolerated and induced long-term anti-tumor immunity, including complete responses (up to 40%) in DMG preclinical models. Mechanistic studies revealed that this therapy increased tumor proliferating T lymphocytes and proinflammatory myeloid cells, including mature dendritic cells with superior tumor antigen uptake capacity. Moreover, the lack of cross-priming DCs and the prevention of DCs recruitment into the tumor abolish the Delta-24-RGD+anti-CD40 anti-tumor effect. This approach shows potential for combining virotherapy with activating antigen-presenting cells in these challenging tumors.
Published in
Targeting the CD40 costimulatory receptor to improve virotherapy efficacy in diffuse midline gliomas
Labiano S, Marco-Sanz J, Ausejo-Mauleon I et al. · Cell reports. Medicine 2025 · PMID 40578365 · doi:10.1016/j.xcrm.2025.102204
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE292938_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1242194 and SRA study SRP573182. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 24 more — browse all 24 samples with per-sample file links →

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