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20S proteasome-regulated proteostasis in ELVAs is critical for oocyte-to-embryo transition and female fertility

GSE293047 Mus musculus (mouse) RNA-seq (gene expression) 16 samples Submitted 2026/04/27 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 16 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
16 samples: 4 × Mus musculus, oocyte, wildtype, GV, biol vs 4 × Mus musculus, oocyte, Psma7 cKO, GV, biol vs 4 × Mus musculus, oocyte, wildtype, MII, biol vs 4 × Mus musculus, oocyte, Psma7 cKO, MII, biolGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
20S proteasome-regulated proteostasis in ELVAs is critical for oocyte-to-embryo transition and female fertilityThe EMBO journal · 2026
Summary (written by the submitters)
Programmed degradation of maternal proteins is essential for the oocyte-to-embryo transition (OET). While pharmacological inhibition studies have established the importance of proteasomes in ovarian reserve maintenance, oocyte maturation and fertilization, the physiological impact of intrinsic proteasome insufficiency and underlying molecular mechanisms remain poorly understood.
Read the full summary ↓
In mice, endolysosomal vesicular assemblies (ELVAs), specialized membraneless compartments composed of proteasomes, endolysosomes and autophagosomes, facilitate protein degradation during oocyte maturation and early embryogenesis. In this study, we generated mice with oocyte-specific deletion of the proteasomal core subunit Psma7, to investigate the physiological function of the 20S proteasome and its roles in ELVAs-mediated protein degradation. PSMA7-deficiency destabilized 20S proteasomes and disrupted translocation of ELVAs, leading to pronounced accumulation of ubiquitinated proteins in oocytes and zygotes. Consequently, maternal Psma7 deletion resulted in female infertility, manifested by impaired oocyte maturation and developmental arrest at one- to two-cell stage. Furthermore, we observed reduced proteasome abundance and dysfunction of ELVAs in aged oocytes, providing a mechanistic explanation for the decline in developmental competence associated with oocyte aging. Taken together, our findings elucidate the critical function of proteasome-regulated proteostasis within ELVAs in maintaining oocyte quality during OET and reproductive aging.
Published in
20S proteasome-regulated proteostasis in ELVAs is critical for oocyte-to-embryo transition and female fertility
Rong Y, Chen Y, Cao H et al. · The EMBO journal 2026 · PMID 42168604 · doi:10.1038/s44318-026-00813-0
This dataset
Open GSE293047 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE293047_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1242683 and SRA study SRP573499. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42168604) ↗
Study design
4 × Mus musculus, oocyte, wildtype, GV, biol vs 4 × Mus musculus, oocyte, Psma7 cKO, GV, biol vs 4 × Mus musculus, oocyte, wildtype, MII, biol vs 4 × Mus musculus, oocyte, Psma7 cKO, MII, biol

Supports a between-group comparison across 16 samples.

How we read this: 4 replicated groups read from 16 sample titles; they account for 16 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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