GEO series

The constitutive 20S proteasome is required for the maintenance and differentiation of spermatogonia in mice

GSE296214 Mus musculus (mouse) Single-cell RNA-seq 2 samples Submitted 2025/05/20 Platform GPL24247

At a glance

Single-cell RNA-seq · mouse · 2 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
Organism: Mus musculus (mouse)
Samples: 2 samples
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: The constitutive 20S proteasome is required for the maintenance and differentiation of spermatogonia in miceJournal of advanced research · 2026

Summary (written by the submitters)

Proteasomes undergo dynamic changes in their types and activities during mammalian spermatogenesis. While the spermatogenesis-specific 20S proteasome (s20S), characterized by PSMA8 substitution of PSMA7, is known to be essential for meiosis I completion, the functions of the constitutive PSMA7-containing 20S proteasomes (c20S) in spermatogenesis remain poorly understood.

Read the full summary ↓

Here, we show that c20S proteasomes are required for the maintenance and differentiation of spermatogonia. PSMA7 is ubiquitously expressed in male germ cells beginning at the early spermatogonial stage, preceding PSMA8 expression. Conditional ablation of Psma7 using Stra8-Cre impairs proteasomal degradation in differentiating spermatogonia, leading to male infertility. Single-cell RNA sequencing analysis reveals that PSMA7-deleted germ cells are arrested at the differentiating spermatogonia stage and fail to enter meiosis. Notably, sufficient overexpression of PSMA8 restores normal spermatogenesis in Psma7-null germ cells, suggesting a potential complementarity of s20S to c20S. Therefore, our results add critical insights into the complex regulation of proteasomal degradation during spermatogenesis.

Published in

The constitutive 20S proteasome is required for the maintenance and differentiation of spermatogonia in mice

Fang A, Cao H, Zhang Y et al. · Journal of advanced research 2026 · PMID 41167419 · doi:10.1016/j.jare.2025.10.068

This dataset

Open GSE296214 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296214_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1258215 and SRA study SRP582904. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41167419) ↗

Samples in this study

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+ 2 more — browse all 2 samples with per-sample file links →

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