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A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts

GSE293090 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2025/03/27 Platform GPL32159
Summary
Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.
Published in
A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts
Bahr J, Poschmann G, Jungmann A et al. · Communications biology 2025 · PMID 40301568 · doi:10.1038/s42003-025-08083-y
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Also filed as BioProject PRJNA1242782 and SRA study SRP573766. Searching any of these in the dataset finder brings you back here.

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