Transcriptomic profiling of human trophoblast stem cells (hTSCs) and HTR-8/SVneo cells under normal and MYBL2 knockdown conditions
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 3 × hTSCs-shNC vs 3 × hTSCs-shMYBL2 vs 3 × HTR8-shNC vs 3 × HTR8-shMYBL2Groups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Dysregulation of MYBL2 impairs extravillous trophoblast lineage development and function, contributing to recurrent spontaneous abortionProceedings of the National Academy of Sciences of the United States of America · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE293321_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1244012 and SRA study SRP575147. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8880323 hTSCs-shNC-1 hTSCs-shNC
- GSM8880324 hTSCs-shNC-2 hTSCs-shNC
- GSM8880325 hTSCs-shNC-3 hTSCs-shNC
- GSM8880326 hTSCs-shMYBL2-1 hTSCs-shMYBL2
- GSM8880327 hTSCs-shMYBL2-2 hTSCs-shMYBL2
- GSM8880328 hTSCs-shMYBL2-3 hTSCs-shMYBL2
- GSM8880329 HTR8-shNC-1 HTR8-shNC
- GSM8880330 HTR8-shNC-2 HTR8-shNC
- GSM8880331 HTR8-shNC-3 HTR8-shNC
- GSM8880332 HTR8-shMYBL2-1 HTR8-shMYBL2
- GSM8880333 HTR8-shMYBL2-2 HTR8-shMYBL2
- GSM8880334 HTR8-shMYBL2-3 HTR8-shMYBL2
- GSE287691 Effects of exposure to the trichloroethylene metabolite S-(1,2-dichlorovinyl)-L-cysteine on HTR-8/SVneo differential gene expression under hypoxic vs. normoxic conditions (O2 < 2%) 16 samples shares htr-8, conditions, svneo
- GSE316921 Effects of SLC2A1 knockdown in intrahepatic cholangiocarcinoma (ICC) cancer-associated fibroblasts (CAFs) on endothelial sprouting under normal and hypoxic conditions 12 samples shares conditions, knockdown, normal
- GSE285142 Effect of knocking down CERS2 or a double knockdown (KD) CERS5/6 in ventricular cardiomyocytes on gene expression under conditions of hypertrophy induced by phorbol 12-myristate 13-acetate (PMA) or controls. 24 samples shares conditions, knockdown
- GSE272427 SWI/SNF complex ATPase activity supports normal syncytiotrophoblast differentiation and shields from EVT differentiation [ATAC-seq] 20 samples shares trophoblast, normal
- GSE305485 Cell cycle regulator MYBL2 is a distinct vulnerability in acute myeloid leukemia 12 samples shares mybl2
- GSE286345 Sex-specific transcriptional differences in Gulf War Illness patients under stressful conditions 242 samples shares conditions
- GSE330188 Crnic Institute Human Trisome Project - Tofacitinib for Immune Skin Conditions in Down Syndrome: PolyA RNAseq from whole blood 194 samples shares conditions
- GSE243579 iPSC-based modeling of preeclampsia identifies defects in extravillous trophoblast differentiation 144 samples shares trophoblast
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.