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Spatial and Single-cell Transcriptomic Characterization of WIF1-induced Normal-tension Glaucoma [Single Cell Multiome ATAC + Gene Expression]

GSE293381 Mus musculus Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing 6 samples Submitted 2025/05/09 Platform GPL24247
Summary
To explore the potential roles of WIF1 upregulation in glaucoma pathogenesis, we performed WIF1 overexpression in mouse retina via AAV delivery and then assessed its impact on IOP, retinal visual function, and RGC survival. We found it causes visual function defects and RGC loss, especially the RGCs from periphery retina. Our integrative analyses from single cell RNA-seq, ATAC-seq, and spatial transcriptomics have further uncovered the dynamic signaling changes from various retinal cells to RGCs. And our spatial transcriptomics results have also revealed the various changes of central and peripheral RGCs after WIF1 overexpression.
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Also filed as BioProject PRJNA1244320 and SRA study SRP575410. Searching any of these in the dataset finder brings you back here.

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