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Mouse digit fibroblast AAV gene delivery platform regulates regenerative outcome

GSE293537 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/12/16 Platform GPL19057
Summary
The mouse digit tip robustly regenerates post-amputation, a process facilitated by the formation of a blastema. Fibroblasts constitute most of this structure, with specific subtypes contributing significantly more to the blastema. However, it remains unclear whether these fibroblast subpopulations and their associated genes are unique to regeneration or part of a broader wound-healing response. In this study, we performed single-cell RNA sequencing of non-regenerating digits to computationally identify fibroblast subpopulations and genes associated with regeneration and fibrosis. Furthermore, we developed a robust adeno-associated virus (AAV)-based gene delivery system to functionally assess the impact of newly identified candidate pro-fibrotic and pro-regenerative genes on wound healing outcomes.
Published in
Mouse digit AAV gene delivery into fibroblasts regulates regenerative outcome
Jou V, Semelsberger SD, Smerczynski J et al. · Science advances 2026 · PMID 41499500 · doi:10.1126/sciadv.adz0229
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Also filed as BioProject PRJNA1244891 and SRA study SRP575728. Searching any of these in the dataset finder brings you back here.

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