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An organoid model of the human adrenal cortex identifies drivers of steroidogenesis and zonation [scRNA-seq]

GSE293781 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2026/03/12 Platform GPL24676
Summary
The adrenal cortex is the main source of steroid hormones including aldosterone, cortisol and androgens. These hormones play a crucial part in maintaining blood pressure, regulating metabolism and sexual development, respectively. The growth factors and transcriptional networks governing adrenal zonation remain poorly understood. Here, we established organoids derived from primary developing human adrenal cortex tissue. These tissue-resident stem cell-derived organoids display longevity and are composed of mature cells producing different steroid hormones. We use spatial transcriptomics to identify zonated growth factors in tissue, and explore the role of 42 different molecules in controlling cell identity and steroidogenesis using organoids. We find that a combination of WNT, cAMP, BMP/TGFβ and nuclear receptors can regulate cell states representing the different cortical zones. Together, human adrenal cortex organoids represent a complex cellular model to study adrenal biology and disease.
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Direct links to NCBI, no account and no request form: the whole study as GSE293781_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1246210 and SRA study SRP576404. Searching any of these in the dataset finder brings you back here.

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