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Binding of KAP1 organizes HP1alpha for transcriptional silencing [ChIP-seq]

GSE293913 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/06/02 Platform GPL19057
Summary
Constitutive heterochromatin is enriched in repetitive elements, including endogenous retroviral element (ERV) sequences that must be repressed to sustain normal cell developmental programs and prevent chromosomal instability. ERVs are silenced through trimethylation of lysine 9 on histone H3 (H3K9me3) by ESET (also known as SETDB1, SET domain bifurcated 1, or KMT1E) and a co-repressor complex containing KAP1 (KRAB-associated protein 1, also known as tripartite motif-containing protein 28, Trim28) in mouse embryonic stem cells. H3K9me3 then serves as a scaffold for the recruitment of proteins that maintain ERVs in a transcriptionally silent state, including the HP1 proteins that themselves interact with the co-repressor KAP1. In this study, we provide structural insights into the KAP1-HP1 interaction. Further, we demonstrate that this interaction plays a role in maintaining inaccessible chromatin at specific ERV elements in embryonic stem cells (ESCs).
Published in
The HP1 box of KAP1 organizes HP1α for silencing of endogenous retroviral elements in embryonic stem cells
Gaurav N, O'Hara R, Hyder U et al. · Nature communications 2025 · PMID 40450002 · doi:10.1038/s41467-025-60279-2
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Direct links to NCBI, no account and no request form: the whole study as GSE293913_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1247409 and SRA study SRP577038. Searching any of these in the dataset finder brings you back here.

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