GEO series
RNA-seq of endothelial cells in wild-type and Shb iECKO mice
GSE293931
Mus musculus
Expression profiling by high throughput sequencing
21 samples
2025/09/17
GPL24247
Summary
E0771.lmb tumors were grown after tamoxifen injection as previously described in wild-type and Shb iECKO (Shb flox/flox; Cdh5-CreERt2) mice, alternatively Shb ICKO (Shb flox/flox; Vav1-cre) mice. Mice were sacrificed on day 14-16 after tumor cell injection. The tumors were digested, CD45+-cells isolated by MACS microbeads (Miltenyi, Bergich Gladbach, Germany) and subsequently CD31+-cells isolated by fluorescence-activated cell sorting (FACS) after staining for CD31. RNA from EC CD31+-cells were subjected to bulk RNAseq.
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