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Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [bulk RNA-seq]

GSE294011 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2025/04/08 Platform GPL23227
Summary
Little is known about the adenogenesis of human endometrial glands. This study explored endometrial adenogenesis using a novel three-dimensional endometrial assembloid model that integrates human endometrial organoids (EOs) and human endometrial stromal cells (HESCs). The model effectively recapitulated endometrial tubular gland formation, underscoring the critical role of stromal-epithelial interactions. Transcriptomic analyses identified WNT7B as a key intrinsic regulator for EO-derived tubular gland formation, which is extrinsically regulated by TGFβ1-VDR interaction between HESCs and EOs. Estradiol stimulated endometrial gland development via WNT7B downregulation in EOs. This finding was validated in an estradiol-stimulated mouse model and clinical samples from women undergoing in vitro fertilization (IVF) cycles. Uterine-specific WNT7B knockout in mice further confirmed its inhibitory role in endometrial gland development. This study offers insights into endometrial adenogenesis and potential therapeutic targets for related endometrial disorders.
Published in
Endometrial Assembloid Model Reveals Endometrial Gland Development Regulation by Estradiol-Driven WNT7B Suppression
Li X, Guo Y, Li J et al. · Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 · PMID 41431163 · doi:10.1002/advs.202509664
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Direct links to NCBI, no account and no request form: the whole study as GSE294011_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1247690 and SRA study SRP577082. Searching any of these in the dataset finder brings you back here.

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