GEO series

PAN2 Maintains mRNA PolyA Tail Homeostasis and Regulates Translation during Spermiogenesis in Mouse

GSE294418 Mus musculus (mouse) RNA-seq (gene expression) 12 samples Submitted 2026/02/06 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 12 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 12 samples: 3 × WT, MII, vs 3 × KO, MII, vs 3 × WT, RS, vs 3 × KO, RS,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: PAN2 maintains mRNA poly(A) tail homeostasis and regulates translation during spermiogenesis in miceNature communications · 2026

Summary (written by the submitters)

Elongation and shortening of the polyA tail are the most common type of post-transcriptional mRNA regulation in eukaryotic cells. Two protein complexes, PAN2-PAN3 and CCR4-NOT, are major cytoplasmic deadenylases that trim the mRNA polyA tails. While PAN2-PAN3 removes the distal portion of the long polyA tails, CCR4-NOT removes adenosine closer to the 3'-UTR.

Read the full summary ↓

Some subunits in the CCR4-NOT complex have been shown to play synergistic biochemical functions during mouse germ cell development. However, the in vivo functions of the upstream deadenylase PAN2-PAN3 remain unclear, particularly in mammals. In this study, we found that germline-specific deletion of Pan2 leads to male infertility. Pan2 knockout did not affect the prophase of meiosis I, but led to abnormal spermiogenesis and apoptosis of male germ-cells. By Palso-seq and Ribo-lite, we found that the deletion of Pan2 increased the number of long-tail mRNAs in spermiogenesis and disrupted the translation efficiency of genes that should be highly translated at this stage. These findings highlight the crucial physiological functions of PAN2 in gametogenesis and expand our understanding of the posttranscriptional regulation of mRNAs in specific physiological processes.

Published in

PAN2 maintains mRNA poly(A) tail homeostasis and regulates translation during spermiogenesis in mice

Wu X, Wu YK, Jia MY et al. · Nature communications 2026 · PMID 41714623 · doi:10.1038/s41467-026-69639-y

This dataset

Open GSE294418 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294418_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1249419 and SRA study SRP577835. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41714623) ↗

Study design

3 × WT, MII, vs 3 × KO, MII, vs 3 × WT, RS, vs 3 × KO, RS,

Supports a between-group comparison across 12 samples.

How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8905127 WT, MII, 1 WT, MII,
GSM8905128 WT, MII, 2 WT, MII,
GSM8905129 WT, MII, 3 WT, MII,
GSM8905130 KO, MII, 1 KO, MII,
GSM8905131 KO, MII, 2 KO, MII,
GSM8905132 KO, MII, 3 KO, MII,
GSM8905133 WT, RS, 1 WT, RS,
GSM8905134 WT, RS, 2 WT, RS,
GSM8905135 WT, RS, 3 WT, RS,
GSM8905136 KO, RS, 1 KO, RS,
GSM8905137 KO, RS, 2 KO, RS,
GSM8905138 KO, RS, 3 KO, RS,

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