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Polysome profiling of mRNA translation in neuroblastoma cells in response to asparagine/alanine supplement

GSE294552 Homo sapiens Expression profiling by high throughput sequencing 6 samples Submitted 2025/05/30 Platform GPL20795
Summary
Neuroblastoma, the most common extracranial solid malignancy in infants and young children, originates from aberrant neural crest cells and accounts for 10-15% of childhood cancer deaths. However, the underlying mechanisms regulating neuroblastoma progression remain to be determined. To investigate the mechanisms regulating tumorigenesis and aggressiveness, we employed the Illumina HiSeq X Ten as a discovery platform to analyze the polysome profiling of mRNA translation in neuroblastoma cells in response to asparagine/alanine (Asn/Ala) supplement. The results showed that 618 and 566 translating mRNAs were respectively elevated or reduced in SK-N-BE(2) after Asn/Ala treatment. Furthermore, we validated the polysome profiling results by western blot with high identity. Overall, our results provided fundamental information about the mRNA translation changes in response to Asn/Ala treatment in human neuroblastoma cells, and these findings will help us understand the pathogenesis of neuroblastoma progression.
Published in
Targeting the Exonic Circular OGT RNA/O-GlcNAc Transferase/Forkhead Box C1 Axis Inhibits Asparagine- and Alanine-Mediated Ferroptosis Repression in Neuroblastoma Progression
Li Q, Cheng Y, Yang C et al. · Research (Washington, D.C.) 2025 · PMID 40416363 · doi:10.34133/research.0703
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Also filed as BioProject PRJNA1250488 and SRA study SRP578420. Searching any of these in the dataset finder brings you back here.

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