GEO series
A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophy
GSE294765
Mus musculus (mouse)
RNA-seq (gene expression)
35 samples
Submitted 2026/04/23
Platform GPL30172Platform GPL24247
At a glance
RNA-seq (gene expression) · mouse · 35 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 35 samples: 27 distinct titles, 8 of them repeatedGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- A skeletal muscle atlas shows neuromuscular junction adaptations to growth and atrophyDevelopmental cell · 2026
Summary (written by the submitters)
The molecular mechanisms underlying the opposing states of muscle atrophy (such as with disuse or in aging) and hypertrophy (such as with increased physical activity) are still not well-defined. Progress has been slowed by challenges in characterizing the niche heterogeneity of skeletal muscle (whereby myofibers are the most abundant) and in obtaining well-characterized samples.
Read the full summary ↓
Here we describe: i) changes in muscle cell populations that are undergoing atrophy or hypertrophy, with complex, opposing cell transcriptional responses during each process; and ii) a remarkable remodeling of the neuromuscular junction (NMJ) domain, whereby we identify emerging new subpopulations of synaptic myonuclei (SynM) and terminal Schwann cells (tSCs) that are specifically adapted to each growth/atrophy condition. High-resolution 3D imaging and spatial transcriptomics reveal these structural and molecular adaptations at the tissue level. Critically, these changes also occur in the NMJ of both denervated and exercised human muscle. Our study lays the ground for identifying novel targets for medical, pharmacological and lifestyle interventions in aging and disease.
Published in
Campanario S, Grima-Terrén M, Rommelfanger M et al.
· Developmental cell 2026
· PMID 42019489
· doi:10.1016/j.devcel.2026.03.010
This dataset
Download · which file do I need?
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
1 · Just have a look
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
2 · Re-analyse it
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294765_RAW.tar (it can run to tens of gigabytes).
3 · Start from the raw reads
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1251572 and SRA study SRP579033. Searching any of these in the dataset finder brings you back here.
Study design
27 distinct titles, 8 of them repeated
Muscle, single-nuclei, 3d post-denervation, B… ×2
Muscle, single-nuclei, 7d post-overloading, B… ×2
Muscle, single-nuclei, 3d post-denervation, B… ×2
Muscle, single-nuclei, 3d post-overloading, B… ×2
Muscle, single-nuclei, 7d post-denervation, B… ×2
Muscle, single-nuclei, 7d post-overloading, B… ×2
Muscle, single-nuclei, 7d post-overloading, B… ×2
Muscle, single-nuclei, basal, Batch Exp2, Chip ×2
How we read this: Read from 35 sample titles: 27 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
Samples in this study
- GSM8919204 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A, R1 (CMO)
- GSM8919205 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A, R1 (GEX)
- GSM8919206 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A, R2 (CMO)
- GSM8919207 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A, R2 (GEX)
- GSM8919208 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A2, R1 (CMO)
- GSM8919209 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A2, R1 (GEX)
- GSM8919210 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A2, R2 (CMO)
- GSM8919211 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A2, R2 (GEX)
- GSM8919212 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A2, R3 (CMO)
- GSM8919213 Muscle, single-cell, basal, 3d, and 7d post-overloading and post-denervation, Batch A2, R3 (GEX)
- GSM8919214 Muscle, single-nuclei, basal, Batch Exp1, R1
- GSM8919215 Muscle, single-nuclei, 3d post-denervation, Batch Exp1, R1
- GSM8919216 Muscle, single-nuclei, 3d post-denervation, Batch Exp1, R2
- GSM8919217 Muscle, single-nuclei, 3d post-overloading, Batch Exp1, R1
- GSM8919218 Muscle, single-nuclei, 7d post-overloading, Batch Exp1, R1
- GSM8919219 Muscle, single-nuclei, 7d post-overloading, Batch Exp1, R2
- GSM8919220 Muscle, single-nuclei, 3d post-denervation, Batch Exp2, Chip1
- GSM8919221 Muscle, single-nuclei, 3d post-denervation, Batch Exp2, Chip2
- GSM8919222 Muscle, single-nuclei, 3d post-overloading, Batch Exp2, Chip2, R1
- GSM8919223 Muscle, single-nuclei, 3d post-overloading, Batch Exp2, Chip1
- GSM8919224 Muscle, single-nuclei, 3d post-overloading, Batch Exp2, Chip2, R2
- GSM8919225 Muscle, single-nuclei, 7d post-denervation, Batch Exp2, Chip1
- GSM8919226 Muscle, single-nuclei, 7d post-denervation, Batch Exp2, Chip2
- GSM8919227 Muscle, single-nuclei, 7d post-overloading, Batch Exp2, Chip1, R1
- GSM8919228 Muscle, single-nuclei, 7d post-overloading, Batch Exp2, Chip2, R1
- GSM8919229 Muscle, single-nuclei, 7d post-overloading, Batch Exp2, Chip1, R2
- GSM8919230 Muscle, single-nuclei, 7d post-overloading, Batch Exp2, Chip2, R2
- GSM8919231 Muscle, single-nuclei, basal, Batch Exp2, Chip1
- GSM8919232 Muscle, single-nuclei, basal, Batch Exp2, Chip2
- GSM8919242 Muscle, visium, 7d post-denervation, A1
- GSM8919243 Muscle, visium, 7d post-denervation, B1
- GSM8919244 Muscle, visium, sham, C1
- GSM8919245 Muscle, visium, sham, D1
- GSM8919246 Muscle, visium, 7d post-overloading, C1
- GSM8919247 Muscle, visium, 7d post-overloading, D1
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Share this dataset
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