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A cellular anatomy of normal adult human female urethra

GSE294771 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/04/15 Platform GPL24676
Summary
A cellular atlas of healthy human female urethra could provide a better mechanistic understanding of voiding dysfunction and infection. Single cell RNA sequencing was performed on young adult human female urethra with in situ validation of putative cell types. The proximal urethra and dorsal ducts were shown to contain a transitional epithelium with basal, intermediate and luminal layers as well as rare neuroendocrine epithelia. Squamous epithelium was predominantly found in the distal urethra. Novel fibroblast subtypes were validated in situ as displaying regional localization with insight into putative functions. Finally, a comparison of male prostatic urethra and female urethra was performed using both single cell and spatial transcriptomics. These data provide a cellular and molecular landscape of male and female urethra to be used as a reference for understanding disease.
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Direct links to NCBI, no account and no request form: the whole study as GSE294771_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1251587 and SRA study SRP579037. Searching any of these in the dataset finder brings you back here.

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