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Single-cell RNA sequencing on peripheral blood PBMCs from 4T1 mouse model treated with STING agonist deliveredy by PEG biopsy marker does not reveal peripheral T cell clonal expansion.

GSE294846 Mus musculus Other; Expression profiling by high throughput sequencing 4 samples Submitted 2025/04/21 Platform GPL34328
Summary
To explore whether immune escape could be secondary to a failure of STING agonist to induce T-cell clonality and expansion, we performed single-cell RNA sequencing (scRNAseq) and T-cell receptor sequencing (TCR-seq) on peripheral PBMCs from animals treated with PEG markers with PBS or 100 µg ADU-S100, using the low tumor burden model of 4T1. Cell types were clustered based on gene signature analysis with 9 resultant clusters. We then analyzed the frequency of unique clonotypes that were expanded following PBS versus ADU-S100 treatment. In both groups, most of the cells that had a unique clonotype did not expand, showing a frequency of 1. There were no specific clonotypes that had a frequency over 5, indicating that ADU-S100 treatment of 4T1 mice does not result in peripheral T cell clonal expansion.
Published in
Immune targeting of triple-negative breast cancer through a clinically actionable STING agonist-CAR T cell platform
Zhang Y, Li Z, Ritter J et al. · Cell reports. Medicine 2025 · PMID 40543510 · doi:10.1016/j.xcrm.2025.102198
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Also filed as BioProject PRJNA1251680 and SRA study SRP579121. Searching any of these in the dataset finder brings you back here.

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