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Cut&Tag profiling of NR1D1 in primary mouse vascular smooth muscle cells with or without vasostatin-2 stimulation

GSE295217 Mus musculus Genome binding/occupancy profiling by high throughput sequencing 4 samples Submitted 2025/09/01 Platform GPL24247
Summary
NR1D1 (also known as Rev-erbα) is a nuclear receptor involved in circadian rhythm and metabolic regulation. Its role in vascular biology is not fully understood. We performed CUT&Tag (Cleavage Under Targets and Tagmentation) to investigate the genome-wide binding profile of the transcription factor NR1D1 in primary vascular smooth muscle cells (VSMCs) isolated from mice. Two experimental conditions were included: cells treated with vasostatin-2 and untreated controls. The goal of this study was to identify potential transcriptional regulatory targets of NR1D1 under basal and vasostatin-2-stimulated conditions. CUT&Tag was carried out using a specific antibody against NR1D1, and sequencing was performed on the Illumina NovaSeq 6000 platform.
Published in
Vasostatin-2 attenuates injury-induced neointimal hyperplasia through the ACE2/MasR/PPARγ/NR1D1/Gas1 axis
Chen Q, Liu J, Madonna R et al. · Cardiovascular research 2025 · PMID 41231769 · doi:10.1093/cvr/cvaf192
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Also filed as BioProject PRJNA1253717 and SRA study SRP580252. Searching any of these in the dataset finder brings you back here.

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