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Targeting branched-chain amino acids alleviates pulmonary fibrosis [scRNA-seq]

GSE295427 Mus musculus Expression profiling by high throughput sequencing 3 samples Submitted 2026/03/17 Platform GPL28330
Summary
We previously confirmed that BCAT2 was reduced in TGF-β1-treated mouse and human lung fibroblasts. Knockdown of BCAT2 significantly promoted COL1A1 expression in response to TGF-β1 treatment. However, the roles of BCAT2 on other cell types, changes in cellular states, and cellular interactions are currently unknown. Here, we used single-cell RNA sequencing to analysis healthy mouse lungs (WT_PBS), mouse lungs with bleomycin-induced fibrosis (WT_BLM), and heterozygous BCAT2+/− (BCAT2 Het) mouse lungs treated with BLM (Het_BLM). Notably, we observed a reduced number of epithelial cells in the BCAT2 Het mice (Figure 3M and S3L). In contrast, the fibroblast and macrophage populations were significantly increased in the BCAT2 Het mice compared to WT mice after bleomycin-induced lung injury.
Published in
Dietary intake and BCAA metabolism regulate pulmonary fibrosis through KDM4A-mediated epigenetic remodeling in male mice
Yao J, Fang S, Lei M et al. · Nature communications 2026 · PMID 42045225 · doi:10.1038/s41467-026-72273-3
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Also filed as BioProject PRJNA1254656 and SRA study SRP580883. Searching any of these in the dataset finder brings you back here.

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