RNA-seq of Melanoma cell lines M257, M238, 451Lu, 451LuR treated with PUFA/Fe3+ compared to untreated controls
RNA-seq (gene expression) · human · 48 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Topic
- MelanomaThis series is part of our curated briefing on it.
- Samples
- 48 samples: 16 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- AMP-activated protein kinase-driven lipid droplet dynamics govern melanoma sensitivity to polyunsaturated fatty acid and iron-induced ferroptosisNature communications · 2025
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE295451_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1254699 and SRA study SRP580871. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 40 samples.
How we read this: 16 replicated groups read from 48 sample titles; they account for 40 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8950322 M257 18 h CTRL1 M257 18 h CTRL
- GSM8950323 M257 18 h CTRL2 M257 18 h CTRL
- GSM8950324 M257 18 hCTRL3
- GSM8950325 M257 18 h PUFA/Fe3+ 1 M257 18 h PUFA/Fe3+
- GSM8950326 M257 18 h PUFA/Fe3+ 2 M257 18 h PUFA/Fe3+
- GSM8950327 M257 18 h PUFA/Fe3+ 3 M257 18 h PUFA/Fe3+
- GSM8950328 M238 18 h CTRL1 M238 18 h CTRL
- GSM8950329 M238 18 h CTRL2 M238 18 h CTRL
- GSM8950330 M238 18 hCTRL3
- GSM8950331 M238 18 h PUFA/Fe3+ 1 M238 18 h PUFA/Fe3+
- GSM8950332 M238 18 h PUFA/Fe3+ 2 M238 18 h PUFA/Fe3+
- GSM8950333 M238 18 h PUFA/Fe3+ 3 M238 18 h PUFA/Fe3+
- GSM8950334 451Lu 18 h CTRL1 451Lu 18 h CTRL
- GSM8950335 451Lu 18 h CTRL2 451Lu 18 h CTRL
- GSM8950336 451Lu 18 hCTRL3
- GSM8950337 451Lu 18 h PUFA/Fe3+ 1 451Lu 18 h PUFA/Fe3+
- GSM8950338 451Lu 18 h PUFA/Fe3+ 2 451Lu 18 h PUFA/Fe3+
- GSM8950339 451Lu 18 h PUFA/Fe3+ 3 451Lu 18 h PUFA/Fe3+
- GSM8950340 451LuR 18 h CTRL1 451LuR 18 h CTRL
- GSM8950341 451LuR 18 h CTRL2 451LuR 18 h CTRL
- GSM8950342 451LuR 18 hCTRL3
- GSM8950343 451LuR 18 h PUFA/Fe3+ 1 451LuR 18 h PUFA/Fe3+
- GSM8950344 451LuR 18 h PUFA/Fe3+ 2 451LuR 18 h PUFA/Fe3+
- GSM8950345 451LuR 18 h PUFA/Fe3+ 3 451LuR 18 h PUFA/Fe3+
- GSM8950346 M257 36 h CTRL1 M257 36 h CTRL
- GSM8950347 M257 36 h CTRL2 M257 36 h CTRL
- GSM8950348 M257 36 hCTRL3
- GSM8950349 M257 36 h PUFA/Fe3+ 1 M257 36 h PUFA/Fe3+
- GSM8950350 M257 36 h PUFA/Fe3+ 2 M257 36 h PUFA/Fe3+
- GSM8950351 M257 36 h PUFA/Fe3+ 3 M257 36 h PUFA/Fe3+
- GSM8950352 M238 36 h CTRL1 M238 36 h CTRL
- GSM8950353 M238 36 h CTRL2 M238 36 h CTRL
- GSM8950354 M238 36 hCTRL3
- GSM8950355 M238 36 h PUFA/Fe3+ 1 M238 36 h PUFA/Fe3+
- GSM8950356 M238 36 h PUFA/Fe3+ 2 M238 36 h PUFA/Fe3+
- GSM8950357 M238 36 h PUFA/Fe3+ 3 M238 36 h PUFA/Fe3+
- GSM8950358 451Lu 36 h CTRL1 451Lu 36 h CTRL
- GSM8950359 451Lu 36 h CTRL2 451Lu 36 h CTRL
- GSM8950360 451Lu 36 hCTRL3
- GSM8950361 451Lu 36 h PUFA/Fe3+ 1 451Lu 36 h PUFA/Fe3+
+ 8 more — browse all 48 samples with per-sample file links →
- GSE130956 Investigation of ST8SIA1 expression in icaritin-treated and untreated melanoma cell lines 12 samples same disease briefing · shares untreated, melanoma
- GSE230489 RNA-sequencing of cutaneous melanoma and non-small cell lung cancer biopsies 38 samples same disease briefing · shares melanoma
- GSE100797 Mutational and neoantigen load predict clinical benefit of adoptive T cell therapy in melanoma 25 samples same disease briefing · shares melanoma
- GSE140150 A rare subpopulation of melanoma cells with low expression of metastasis suppressor NME1 has a neural crest-like phenotype and is highly metastatic in vivo 6 samples same disease briefing · shares melanoma
- GSE12391 Melanoma: comparison between common nevi, radial/vertical growth phase melanoma, metastases and dysplastic nevi 114 samples same disease briefing · shares melanoma
- GSE134459 Genome-wide maps of transcriptomic and epigenomic state in melanoma cell lines 73 samples same disease briefing · shares melanoma
- GSE123728 Clinical and Immunologic Activity of a Single Dose of Neoadjuvant PD-1 Blockade and Predictors of Clinical Outcomes in Resectable Melanoma 24 samples same disease briefing · shares melanoma
- GSE91061 Molecular portraits of tumor mutational and micro-environmental sculpting by immune checkpoint blockade therapy 109 samples same disease briefing
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.