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Next Generation Sequencing Facilitates Quantitative Analysis of RBM22-silencing and Control Primary Neonatal Mouse Cardiomyocytes Transcriptomes

GSE295695 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2026/02/12 Platform GPL24247
Summary
Purpose:The purpose of this study is to identify genes that are either activated or silenced in cardiomyocytes treated with siRNA targeting RBM22 (si-Rbm22) compared to control siRNA (si-Control). Gene expression differences between the two samples were identified using transcriptome profiling (RNA-seq) analysis. Methods: Primary neonatal mouse cardiomyocytes were isolated from neonatal C57BL/6J mice at postnatal day 1.Cardiomyocytes were stained to confirm the expression of cTnT. Cells with purity >97.5% were used for subsequent experiments. Neonatal cardiomyocytes in primary culture were transfected with either siRNA targeting Rbm22 (si-Rbm22) or a non-targeting control siRNA (si-Control) for 48 h, of which RNA profiles were generated by deep sequencing using Illumina. Results: We mapped about 10 million sequence reads per sample to the mouse genome and identified numerous genes with significant mRNA variation between cardiomyocytes transfected with the indicated siRNAs.
Published in
Restoration of RBM22 overcomes the transcriptional and epigenetic barriers of cardiomyocyte proliferation for heart regeneration
Duan X, Tan Y, Zhang Y et al. · Nature communications 2026 · PMID 41803140 · doi:10.1038/s41467-026-70235-3
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Also filed as BioProject PRJNA1255614 and SRA study SRP581333. Searching any of these in the dataset finder brings you back here.

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