EBF1 and PAX5 exert dose dependent epigenetic repression of T-lineage potential [RNA-seq]
Single-cell RNA-seq · mouse · 20 samples
- What it is
- Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart. Read from the summary: GEO's own type field only says 'expression profiling by sequencing', so confirm it in the paper.
- Organism
- Mus musculus (mouse)
- Samples
- 20 samples: 6 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- B-lineage commitment is dependent on a reversible epigenetic switchGenes & development · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE295900_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.
Also filed as BioProject PRJNA1256753 and SRA study SRP582076. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 20 samples.
How we read this: 6 replicated groups read from 20 sample titles; they account for 20 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8960402 BM WT proB, RNA-seq, rep1 BM WT proB, RNA-seq,
- GSM8960403 BM WT proB, RNA-seq, rep2 BM WT proB, RNA-seq,
- GSM8960404 BM WT proB, RNA-seq, rep3 BM WT proB, RNA-seq,
- GSM8960405 BM Ebf1+/- proB, RNA-seq, rep1 BM Ebf1+/- proB, RNA-se…
- GSM8960406 BM Ebf1+/- proB, RNA-seq, rep2 BM Ebf1+/- proB, RNA-se…
- GSM8960407 BM Ebf1+/- proB, RNA-seq, rep3 BM Ebf1+/- proB, RNA-se…
- GSM8960408 BM Pax5+/- proB, RNA-seq, rep1 BM Pax5+/- proB, RNA-se…
- GSM8960409 BM Pax5+/- proB, RNA-seq, rep2 BM Pax5+/- proB, RNA-se…
- GSM8960410 BM Pax5+/- proB, RNA-seq, rep3 BM Pax5+/- proB, RNA-se…
- GSM8960411 BM Ebf1+/-Pax5+/- proB total, RNA-seq, rep1 BM Ebf1+/-Pax5+/- proB …
- GSM8960412 BM Ebf1+/-Pax5+/- proB total, RNA-seq, rep2 BM Ebf1+/-Pax5+/- proB …
- GSM8960413 BM Ebf1+/-Pax5+/- proB total, RNA-seq, rep3 BM Ebf1+/-Pax5+/- proB …
- GSM8960414 BM Ebf1+/-Pax5+/- proB total, RNA-seq, rep4 BM Ebf1+/-Pax5+/- proB …
- GSM8960415 BM Ebf1+/-Pax5+/- proB CD25-, RNA-seq, rep1 BM Ebf1+/-Pax5+/- proB …
- GSM8960416 BM Ebf1+/-Pax5+/- proB CD25-, RNA-seq, rep2 BM Ebf1+/-Pax5+/- proB …
- GSM8960417 BM Ebf1+/-Pax5+/- proB CD25-, RNA-seq, rep3 BM Ebf1+/-Pax5+/- proB …
- GSM8960418 BM Ebf1+/-Pax5+/- proB CD25-, RNA-seq, rep4 BM Ebf1+/-Pax5+/- proB …
- GSM8960419 BM Ebf1+/-Pax5+/- proB CD25+, RNA-seq, rep2 BM Ebf1+/-Pax5+/- proB …
- GSM8960420 BM Ebf1+/-Pax5+/- proB CD25+, RNA-seq, rep3 BM Ebf1+/-Pax5+/- proB …
- GSM8960421 BM Ebf1+/-Pax5+/- proB CD25+, RNA-seq, rep4 BM Ebf1+/-Pax5+/- proB …
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- GSE270728 Analysis of striatal zinc finger protein (ZFP) repression of mutant Huntingtin (HTT) in the zQ175DN knock-in mouse model of Huntington's disease (snRNA-seq) 120 samples shares repression, rna-seq
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.