Genomic Profiling of Sirt6 binding sites in the neuroblastoma cell line ShSy-5y under normal culture conditions.

Histone deacetylases are important regulators of gene expression and chromatin conformation. Sirt6 is a histone deacetylase that regulates gene expression by deacetylating histone H3 on the specific lysine residues K9 and K56. Notably, Sirt6 expression and activity negatively correlate with biological aging and the onset of age-related diseases, such as Alzheimer’s disease. Moreover, Sirt6 also binds to DNA double-strand breaks, as a DSB-sensor protein, and regulates the DNA damage response, promoting non-homologous end-joining (NHEJ) or homologous recombination (HR) pathways. Therefore, Sirt6 functions as a stress signaling epigenetic regulator capable of coordinating the cellular response to several cytotoxic stimuli, including DNA damage, as one of its main regulatory outputs. We performed Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to investigate the Sirt6 genomic-binding profile in the neuroblastoma cell line ShSy-5y under normal culture conditions. This experiment allows the scientific community and us to identify the Sirt6 targets at the gene level, and predict a possible regulatory role on its expression. The study on Sirt6 binding to specific genes was further confirmed and analyzed by ChIP-qPCR under specific experimental conditions.