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RNA sequencing reveals differential MYC-driven gene expression as a driver of a divergent cholangiocyte response to stress

GSE296039 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/07/30 Platform GPL20301
Summary
In Primary Sclerosing Cholangitis (PSC), some cholangiocytes undergo cell cycle arrest (senescence) while others proliferate (ductular reaction). Our aim was to determine the mechanisms driving this divergent response. We used lipopolysaccharide (LPS) to stress normal human cholangiocytes (NHC) transfected with a senescence reporter, p16- promoter driven green fluorescent protein. We performed RNA sequencing to assess gene expression profiles on non-senescent (GFP-) and senescent (GFP+) cholangiocytes. Our analysis revealed a MYC-driven gene expression profile in non-senescent cholangiocytes and suggest that MYC may function as a "molecular switch" in determining cholangiocyte responses to stress.
Published in
Differential MYC Phosphorylation Drives the Divergent Cholangiocyte Response to Stress
O'Hara SP, Splinter PL, Felzen A et al. · Cellular and molecular gastroenterology and hepatology 2025 · PMID 40449843 · doi:10.1016/j.jcmgh.2025.101547
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Also filed as BioProject PRJNA1257369 and SRA study SRP582480. Searching any of these in the dataset finder brings you back here.

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